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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2012.04.004
|2 doi
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|a pubmed24n0725.xml
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|a (DE-627)NLM217733751
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|a (NLM)22579939
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Lu, Hsi-Chi
|e verfasserin
|4 aut
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|a Cloning and expression of pathogenesis-related protein 4 from jelly fig (Ficus awkeotsang Makino) achenes associated with ribonuclease, chitinase and anti-fungal activities
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 27.09.2012
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a GENBANK: ADO24163
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|a Citation Status MEDLINE
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|a Copyright © 2012 Elsevier Masson SAS. All rights reserved.
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|a A cDNA fragment (FaPR4) encoding a class I pathogenesis-related protein 4 (PR-4) from Ficus awkeotsang was obtained by PCR cloning. Plant PR-4s were grouped into class I and II, differing by the presence of ChtBD and hinge. The predicted mature FaPR4 comprises N-terminal chitin-binding domain (ChtBD), hinge, Barwin domain and C-terminal extension. FaPR4-C, an N-terminal truncated form of FaPR4, was designed to mimic the structural feature of class II PR-4s. FaPR4 and FaPR4-C were over-expressed in yeast Pichia pastoris, and both recombinants exhibited RNase and anti-fungal activities. To our knowledge, it is the first report that FaPR4, a member of class I PR-4s has RNase activity as class II. FaPR4 possesses better anti-fungal activities toward Fusarium oxysporum and Sclerotium rolfsii than FaPR4-C. Heat-treated FaPR4 remained RNase and anti-fungal activities; while heat-treated FaPR4-C lost those activities. Therefore, ChtBD of FaPR4 may not only contribute to its anti-fungal but also improve the thermal stability of protein. It also implied the correlation of RNase activity with anti-fungal activity of FaPR4-C. Furthermore, FaPR4 was detected to have weak but significant chitinase activity, and its chitinase activity was reduced after heat treatment. The chitinase activity by FaPR4-C was much lower than FaPR4
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Antifungal Agents
|2 NLM
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|a DNA, Complementary
|2 NLM
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|a Plant Proteins
|2 NLM
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|a pathogenesis-related proteins, plant
|2 NLM
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|a Chitin
|2 NLM
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|a 1398-61-4
|2 NLM
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| 650 |
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|a RNA
|2 NLM
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|a 63231-63-0
|2 NLM
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|a Ribonucleases
|2 NLM
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| 650 |
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|a EC 3.1.-
|2 NLM
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| 650 |
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|a Chitinases
|2 NLM
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| 650 |
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|a EC 3.2.1.14
|2 NLM
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| 700 |
1 |
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|a Lin, Jia-Hui
|e verfasserin
|4 aut
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1 |
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|a Chua, Anna C N
|e verfasserin
|4 aut
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|a Chung, Tse-Yu
|e verfasserin
|4 aut
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| 700 |
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|a Tsai, I-Chun
|e verfasserin
|4 aut
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| 700 |
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|a Tzen, Jason T C
|e verfasserin
|4 aut
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|a Chou, Wing-Ming
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 56(2012) vom: 05. Juli, Seite 1-13
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:56
|g year:2012
|g day:05
|g month:07
|g pages:1-13
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|u http://dx.doi.org/10.1016/j.plaphy.2012.04.004
|3 Volltext
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|d 56
|j 2012
|b 05
|c 07
|h 1-13
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