Interactions of endoglucanases with amorphous cellulose films resolved by neutron reflectometry and quartz crystal microbalance with dissipation monitoring

A study of the interaction of four endoglucanases with amorphous cellulose films by neutron reflectometry (NR) and quartz crystal microbalance with dissipation monitoring (QCM-D) is reported. The endoglucanases include a mesophilic fungal endoglucanase (Cel45A from H. insolens), a processive endoglu...

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Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1992. - 28(2012), 22 vom: 05. Juni, Seite 8348-58
1. Verfasser:	Cheng, Gang (VerfasserIn)
Weitere Verfasser:	Datta, Supratim, Liu, Zelin, Wang, Chao, Murton, Jaclyn K, Brown, Page A, Jablin, Michael S, Dubey, Manish, Majewski, Jaroslaw, Halbert, Candice E, Browning, James F, Esker, Alan R, Watson, Brian J, Zhang, Haito, Hutcheson, Steven W, Huber, Dale L, Sale, Kenneth L, Simmons, Blake A, Kent, Michael S
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2012
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Research Support, U.S. Gov't, Non-P.H.S. Bacterial Proteins Fungal Proteins Ionic Liquids Cellulose 9004-34-6 Cellulase EC 3.2.1.4

Beschreibung
Zusammenfassung:	A study of the interaction of four endoglucanases with amorphous cellulose films by neutron reflectometry (NR) and quartz crystal microbalance with dissipation monitoring (QCM-D) is reported. The endoglucanases include a mesophilic fungal endoglucanase (Cel45A from H. insolens), a processive endoglucanase from a marine bacterium (Cel5H from S. degradans ), and two from thermophilic bacteria (Cel9A from A. acidocaldarius and Cel5A from T. maritima ). The use of amorphous cellulose is motivated by the promise of ionic liquid pretreatment as a second generation technology that disrupts the native crystalline structure of cellulose. The endoglucanases displayed highly diverse behavior. Cel45A and Cel5H, which possess carbohydrate-binding modules (CBMs), penetrated and digested within the bulk of the films to a far greater extent than Cel9A and Cel5A, which lack CBMs. While both Cel45A and Cel5H were active within the bulk of the films, striking differences were observed. With Cel45A, substantial film expansion and interfacial broadening were observed, whereas for Cel5H the film thickness decreased with little interfacial broadening. These results are consistent with Cel45A digesting within the interior of cellulose chains as a classic endoglucanase, and Cel5H digesting predominantly at chain ends consistent with its designation as a processive endoglucanase
Beschreibung:	Date Completed 06.09.2012 Date Revised 05.06.2012 published: Print-Electronic Citation Status MEDLINE
ISSN:	1520-5827
DOI:	10.1021/la300955q