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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1111/j.1365-2761.2012.01363.x
|2 doi
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|a eng
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| 100 |
1 |
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|a Bland, F
|e verfasserin
|4 aut
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|a Development and validation of a range of endogenous controls to support the implementation of practical Taqman real-time PCR-based surveillance for fish diseases within aquaculture
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 27.08.2012
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2012 Crown copyright.
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|a The use of Taqman real-time PCR-based technology has recently become more frequent in the detection of pathogens in the aquaculture industry. This interest has necessitated the development of robust and reliable pathogen-detection assays. The development of a range of endogenous control assays to be run alongside these diagnostic assays works to further increase confidence in the latter. This study describes the design of a range of endogenous control assays based on the elongation factor 1-α (EF1-α) gene specific to a range of fish species including Atlantic salmon, Salmo salar; rainbow trout, Oncorhynchus mykiss; brown trout, Salmo trutta; cod, Gadus morhua; haddock, Melanogrammus aeglefinus; saithe, Pollachius virens; whiting, Merlangius merlangus; Norway pout, Trisopterus esmarkii; carp (family Cyprinidae), roach, Rutilus rutilus; European eel, Anguilla anguilla; and herring, Clupea harengus, as well as a number of fish cell lines. Evidence is provided of the validation of these assays for specific species, a range of tissue types and cell lines as well as an example of the potential uses of these assays
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|a Journal Article
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| 650 |
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4 |
|a Validation Study
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| 650 |
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7 |
|a DNA Primers
|2 NLM
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| 700 |
1 |
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|a McIntosh, R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bain, N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Snow, M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 35(2012), 6 vom: 09. Juni, Seite 447-54
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
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|g volume:35
|g year:2012
|g number:6
|g day:09
|g month:06
|g pages:447-54
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| 856 |
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|u http://dx.doi.org/10.1111/j.1365-2761.2012.01363.x
|3 Volltext
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