Technique for strand-specific gene-expression analysis and monitoring of primer-independent cDNA synthesis in reverse transcription
Primer-independent cDNA synthesis during reverse transcription hinders quantitative analysis of bidirectional mRNA synthesis in eukaryotes as well as in cells infected with RNA viruses. We report a simple RT-PCR-based assay for strand-specific gene-expression analysis. By modifying the cDNA sequence...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 52(2012), 4 vom: 27. Apr., Seite 263-70
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| 1. Verfasser: |
Feng, Lin
(VerfasserIn) |
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| Weitere Verfasser: |
Lintula, Susanna,
Ho, Tho Huu,
Anastasina, Maria,
Paju, Annukka,
Haglund, Caj,
Stenman, Ulf-Håkan,
Hotakainen, Kristina,
Orpana, Arto,
Kainov, Denis,
Stenman, Jakob |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
DNA Primers
DNA, Complementary
PB2 protein, Influenzavirus A
RNA, Viral
Viral Proteins
RNA-Dependent RNA Polymerase
EC 2.7.7.48 |
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