Technique for strand-specific gene-expression analysis and monitoring of primer-independent cDNA synthesis in reverse transcription

Primer-independent cDNA synthesis during reverse transcription hinders quantitative analysis of bidirectional mRNA synthesis in eukaryotes as well as in cells infected with RNA viruses. We report a simple RT-PCR-based assay for strand-specific gene-expression analysis. By modifying the cDNA sequence...

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Publié dans:BioTechniques. - 1991. - 52(2012), 4 vom: 27. Apr., Seite 263-70
Auteur principal: Feng, Lin (Auteur)
Autres auteurs: Lintula, Susanna, Ho, Tho Huu, Anastasina, Maria, Paju, Annukka, Haglund, Caj, Stenman, Ulf-Håkan, Hotakainen, Kristina, Orpana, Arto, Kainov, Denis, Stenman, Jakob
Format: Article en ligne
Langue:English
Publié: 2012
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't DNA Primers DNA, Complementary PB2 protein, Influenzavirus A RNA, Viral Viral Proteins RNA-Dependent RNA Polymerase EC 2.7.7.48