Presence and removal of a contaminating NADH oxidation activity in recombinant maltose-binding protein fusion proteins expressed in Escherichia coli

We observed the presence of contaminating NADH oxidation activity in maltose binding protein (MBP) fusion proteins expressed in Escherichia coli and purified using conventional amylose resin-based affinity chromatography. This contaminating NADH oxidation activity was detectable with at least four d...

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Veröffentlicht in:	BioTechniques. - 1991. - 52(2012), 4 vom: 27. Apr., Seite 247-53
1. Verfasser:	Guo, Fengguang (VerfasserIn)
Weitere Verfasser:	Zhu, Guan
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2012
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, N.I.H., Extramural Acyl Coenzyme A Maltose-Binding Proteins Protozoan Proteins Recombinant Fusion Proteins NAD 0U46U6E8UK Octoxynol 9002-93-1 mehr... crotonyl-coenzyme A 992-67-6 Glycerol PDC6A3C0OX