On-chip synthesis of RNA aptamer microarrays for multiplexed protein biosensing with SPR imaging measurements
Microarrays of RNA aptamers are fabricated in a one-step, multiplexed enzymatic synthesis on gold thin films in a microfluidic format and then employed in the detection of protein biomarkers with surface plasmon resonance imaging (SPRI) measurements. Single-stranded RNA (ssRNA) oligonucleotides are...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 28(2012), 22 vom: 05. Juni, Seite 8281-5
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| 1. Verfasser: |
Chen, Yulin
(VerfasserIn) |
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| Weitere Verfasser: |
Nakamoto, Kohei,
Niwa, Osamu,
Corn, Robert M |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Aptamers, Nucleotide
Biomarkers
VEGFA protein, human
Vascular Endothelial Growth Factor A
Viral Proteins
Gold
7440-57-5
mehr...
DNA
9007-49-2
bacteriophage T7 RNA polymerase
EC 2.7.7.-
DNA-Directed RNA Polymerases
EC 2.7.7.6
Ribonuclease H
EC 3.1.26.4
Thrombin
EC 3.4.21.5 |
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