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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1021/la3004245
|2 doi
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|a pubmed24n0722.xml
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|a DE-627
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|e rakwb
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|a eng
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|a Syamala, Kiran Manikantan
|e verfasserin
|4 aut
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|a Inhibition assay of yeast cell walls by plasmon resonance Rayleigh scattering and surface-enhanced Raman scattering imaging
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 23.10.2012
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We report on plasmon resonance Rayleigh scattering (PRRS) and surface enhanced Raman scattering (SERS) imaging for inhibition assay of yeast cell walls. This assay reveals that the proteins having alkali sensitive linkage bound to β1,3 glucan frameworks in cell walls are involved in SERS activity. The result is further confirmed by comparison of genetically modified cells and wild type cells. Finally, we find that PRRS and SERS spots do not appear on cell walls when daughter cells are enough smaller than parent ones, but appear when size of daughter cells are comparable to parent cells. This finding indicates the relationship between expression of the proteins that generate SERS spots and cell division. These results demonstrate that PRRS and SERS imaging can be a convenient and sensitive method for analysis of cell walls
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|a Journal Article
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|a EC 3.2.1.-
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|a endo-1,6-beta-glucanase
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|a EC 3.2.1.75
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|a Hydrofluoric Acid
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| 700 |
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|a Abe, Hiroko
|e verfasserin
|4 aut
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|a Fujita, Yasuko
|e verfasserin
|4 aut
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|a Tomimoto, Kazuya
|e verfasserin
|4 aut
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|a Biju, Vasudevanpillai
|e verfasserin
|4 aut
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|a Ishikawa, Mitsuru
|e verfasserin
|4 aut
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|a Ozaki, Yukihiro
|e verfasserin
|4 aut
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|a Itoh, Tamitake
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 28(2012), 24 vom: 19. Juni, Seite 8952-8
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|x 1520-5827
|7 nnns
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|g volume:28
|g year:2012
|g number:24
|g day:19
|g month:06
|g pages:8952-8
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