Characterization and expression analysis of six MADS-box genes in maize (Zea mays L.)

Copyright © 2012 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 169(2012), 8 vom: 15. Mai, Seite 797-806
1. Verfasser: Zhang, Zhongbao (VerfasserIn)
Weitere Verfasser: Li, Huiyong, Zhang, Dengfeng, Liu, Yinghui, Fu, Jing, Shi, Yunsu, Song, Yanchun, Wang, Tianyu, Li, Yu
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't DNA-Binding Proteins MADS Domain Proteins Plant Proteins Transcription Factors ZmMEK1 protein, Zea mays
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520 |a MADS-box genes encode a family of transcription factors, which control diverse developmental processes in flowering plants, with organs ranging from roots, flowers and fruits. In this study, six maize cDNAs encoding MADS-box proteins were isolated. BLASTX searches and phylogenetic analysis indicated that the six MADS-box genes belonging to the AGL2-like clade. qRT-PCR analysis revealed that these genes had differential expression patterns in different organs in maize. The results of yeast one-hybrid system indicated that the protein ZMM3-1, ZMM3-2, ZMM6, ZMM7-L, ZMM8-L and ZMM14-L had transcriptional activation activity. Subcellular localization of ZMM7-L demonstrated that the fluorescence of ZMM7-L-GFP was mainly detected in the nuclei of onion epidermal cells. qRT-PCR analysis for expression pattern of ZMM7-L showed that the gene was up-regulated by abiotic stresses and down-regulated by exogenous ABA. The germination rates of over-expression transgenic lines were lower than that of the wild type on medium with 150 mM NaCl, 350 mM mannitol. These results indicated that ZMM7-L might be a negative transcription factor responsive to abiotic stresses 
650 4 |a Comparative Study 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a DNA-Binding Proteins  |2 NLM 
650 7 |a MADS Domain Proteins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a ZmMEK1 protein, Zea mays  |2 NLM 
700 1 |a Li, Huiyong  |e verfasserin  |4 aut 
700 1 |a Zhang, Dengfeng  |e verfasserin  |4 aut 
700 1 |a Liu, Yinghui  |e verfasserin  |4 aut 
700 1 |a Fu, Jing  |e verfasserin  |4 aut 
700 1 |a Shi, Yunsu  |e verfasserin  |4 aut 
700 1 |a Song, Yanchun  |e verfasserin  |4 aut 
700 1 |a Wang, Tianyu  |e verfasserin  |4 aut 
700 1 |a Li, Yu  |e verfasserin  |4 aut 
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