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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2011.12.020
|2 doi
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|a pubmed24n0721.xml
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|a (DE-627)NLM216416590
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|a (NLM)22440334
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Zhang, Zhongbao
|e verfasserin
|4 aut
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|a Characterization and expression analysis of six MADS-box genes in maize (Zea mays L.)
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 09.08.2012
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|a Date Revised 10.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2012 Elsevier GmbH. All rights reserved.
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|a MADS-box genes encode a family of transcription factors, which control diverse developmental processes in flowering plants, with organs ranging from roots, flowers and fruits. In this study, six maize cDNAs encoding MADS-box proteins were isolated. BLASTX searches and phylogenetic analysis indicated that the six MADS-box genes belonging to the AGL2-like clade. qRT-PCR analysis revealed that these genes had differential expression patterns in different organs in maize. The results of yeast one-hybrid system indicated that the protein ZMM3-1, ZMM3-2, ZMM6, ZMM7-L, ZMM8-L and ZMM14-L had transcriptional activation activity. Subcellular localization of ZMM7-L demonstrated that the fluorescence of ZMM7-L-GFP was mainly detected in the nuclei of onion epidermal cells. qRT-PCR analysis for expression pattern of ZMM7-L showed that the gene was up-regulated by abiotic stresses and down-regulated by exogenous ABA. The germination rates of over-expression transgenic lines were lower than that of the wild type on medium with 150 mM NaCl, 350 mM mannitol. These results indicated that ZMM7-L might be a negative transcription factor responsive to abiotic stresses
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA-Binding Proteins
|2 NLM
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|a MADS Domain Proteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Transcription Factors
|2 NLM
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|a ZmMEK1 protein, Zea mays
|2 NLM
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|a Li, Huiyong
|e verfasserin
|4 aut
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|a Zhang, Dengfeng
|e verfasserin
|4 aut
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|a Liu, Yinghui
|e verfasserin
|4 aut
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|a Fu, Jing
|e verfasserin
|4 aut
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|a Shi, Yunsu
|e verfasserin
|4 aut
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|a Song, Yanchun
|e verfasserin
|4 aut
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|a Wang, Tianyu
|e verfasserin
|4 aut
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|a Li, Yu
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 169(2012), 8 vom: 15. Mai, Seite 797-806
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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|g volume:169
|g year:2012
|g number:8
|g day:15
|g month:05
|g pages:797-806
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|u http://dx.doi.org/10.1016/j.jplph.2011.12.020
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