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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2012.01.015
|2 doi
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|a pubmed25n0720.xml
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|a (NLM)22405592
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
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|a An, Xiu-Hong
|e verfasserin
|4 aut
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|a The apple WD40 protein MdTTG1 interacts with bHLH but not MYB proteins to regulate anthocyanin accumulation
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|c 2012
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 22.07.2014
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|a Date Revised 21.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2012 Elsevier GmbH. All rights reserved.
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|a The abundance of anthocyanins and proanthocyanins in apples is tightly regulated by three classes of regulatory factors, MYB, bHLH and WD40 proteins, only some of which have been previously identified. In this study, we identified an apple WD40 protein (MdTTG1) that promotes the accumulation of anthocyanins. The biosynthetic genes required downstream in the flavonoid pathway were up-regulated when MdTTG1 was over-expressed in Arabidopsis. Consistent with its role as a transcriptional regulator, an MdTTG1-GFP fusion protein was observed only in the nucleus. We assayed the expression patterns of this gene in different organs and found that they were positively correlated with anthocyanin accumulation in the apple. Yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that MdTTG1 interacted with bHLH transcription factors (TFs) but not MYB protein, whereas bHLH was known to interact with MYB in apples. However, based on a ChIP assay, MdTTG1 does not appear to bind to the promoter of the anthocyanin biosynthetic genes MdDFR and MdUFGT. Taken together, these results suggest that the apple WD40 protein MdTTG1 interacts with bHLH but not MYB proteins to regulate anthocyanin accumulation
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Anthocyanins
|2 NLM
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|a Basic Helix-Loop-Helix Transcription Factors
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Recombinant Fusion Proteins
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 700 |
1 |
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|a Tian, Yi
|e verfasserin
|4 aut
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|a Chen, Ke-Qin
|e verfasserin
|4 aut
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|a Wang, Xiao-Fei
|e verfasserin
|4 aut
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|a Hao, Yu-Jin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 169(2012), 7 vom: 01. Mai, Seite 710-7
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:169
|g year:2012
|g number:7
|g day:01
|g month:05
|g pages:710-7
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|u http://dx.doi.org/10.1016/j.jplph.2012.01.015
|3 Volltext
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