Photosynthetic performance, lipid production and biomass composition in response to nitrogen limitation in marine microalgae

Copyright © 2012 Elsevier Masson SAS. All rights reserved.

Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 54(2012) vom: 03. Mai, Seite 70-7
Auteur principal: Jiang, Yuelu (Auteur)
Autres auteurs: Yoshida, Tomomi, Quigg, Antonietta
Format: Article en ligne
Langue:English
Publié: 2012
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article Research Support, Non-U.S. Gov't Biofuels Fertilizers Lipids Photosystem II Protein Complex Plant Proteins Nitrogen N762921K75
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520 |a Copyright © 2012 Elsevier Masson SAS. All rights reserved. 
520 |a Increasing energy prices demand a renewable, carbon neutral, transport fuel that is environmentally and commercially sustainable. The interest in the production of microalgae as biofuels is increasing due to their high oil content, rapid biomass production and small foot print. In this research, marine microalgae Dunaliella tertiolecta (Chlorophyceae) and Thalassiosira pseudonana (Bacillariophyceae) were incubated in nitrogen (N)-replete medium, and then transferred to N-free medium for 15 and 11 days, respectively. Fluorescence induction and relaxation (FIRe) fluorometry and Fourier transform infrared spectroscopy (FTIR) were used to monitor the photosynthetic performance, lipid production and metabolic responses to changing N availability. Growth rates of D. tertiolecta and T. pseudonana were 0.84 ± 0.16 d(-1) and 1.21 ± 0.09 d(-), respectively in N-replete medium. Upon transfer to N-free medium. The growth rates of T. pseudonana declined rapidly, while D. tertiolecta continued to grow for 5 days in N-free medium before growth declined slowly. The maximum quantum yield of photochemistry (F(v)/F(m)) remained high initially for D. tertiolecta but decreased immediately after transfer to N-free media for T. pseudonana. The functional absorption cross section for PSII (σ(PSII)) increased, the time constant for Q(A) reoxidation (τ(Qa)) and connectivity factor (p) decreased in parallel to the nutritional status of the microalgae. The relative protein and lipid content varied in response to N limitation, but carbohydrates did not change. Based on FTIR, D. tertiolecta and T. pseudonana produced 20-26% lipid when most stressed. The combination of photosynthetic efficiency and biomass composition monitoring provided evidence that metabolic strategies to changing nutrient status are species-specific 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Biofuels  |2 NLM 
650 7 |a Fertilizers  |2 NLM 
650 7 |a Lipids  |2 NLM 
650 7 |a Photosystem II Protein Complex  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Nitrogen  |2 NLM 
650 7 |a N762921K75  |2 NLM 
700 1 |a Yoshida, Tomomi  |e verfasserin  |4 aut 
700 1 |a Quigg, Antonietta  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 54(2012) vom: 03. Mai, Seite 70-7  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnas 
773 1 8 |g volume:54  |g year:2012  |g day:03  |g month:05  |g pages:70-7 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2012.02.012  |3 Volltext 
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