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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ers006
|2 doi
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|a pubmed24n0719.xml
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|a (DE-627)NLM215838165
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|a (NLM)22378952
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Yang, Lijun
|e verfasserin
|4 aut
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| 245 |
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|a Expression of hypoallergenic Der f 2 derivatives with altered intramolecular disulphide bonds induces the formation of novel ER-derived protein bodies in transgenic rice seeds
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 07.09.2012
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|a Date Revised 10.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a House dust mites (HDM) are the most common source of indoor allergens and are associated with allergic diseases worldwide. To benefit allergic patients, safer and non-invasive mucosal routes of oral administration are considered to be the best alternative to conventional allergen-specific immunotherapy. In this study, transgenic rice was developed expressing derivatives of the major HDM allergen Der f 2 with reduced Der f 2-specific IgE reactivity by disrupting intramolecular disulphide bonds in Der f 2. These derivatives were produced specifically as secretory proteins in the endosperm tissue of seeds under the control of the endosperm-specific glutelin GluB-1 promoter. Notably, modified Der f 2 derivatives aggregated in the endoplasmic reticulum (ER) lumen and were deposited in a unique protein body (PB)-like structure tentatively called the Der f 2 body. Der f 2 bodies were characterized by their intracellular localization and physico-chemical properties, and were distinct from ER-derived PBs (PB-Is) and protein storage vacuoles (PB-IIs). Unlike ER-derived organelles such as PB-Is, Der f 2 bodies were rapidly digested in simulated gastric fluid in a manner similar to that of PB-IIs. Oral administration in mice of transgenic rice seeds containing Der f 2 derivatives encapsulated in Der f 2 bodies suppressed Der f 2-specific IgE and IgG production compared with that in mice fed non-transgenic rice seeds, and the effect was dependent on the type of Der f 2 derivative expressed. These results suggest that engineered hypoallergenic Der f 2 derivatives expressed in the rice seed endosperm could serve as a basis for the development of viable strategies for the oral delivery of vaccines against HDM allergy
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Allergens
|2 NLM
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| 650 |
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7 |
|a Antigens, Dermatophagoides
|2 NLM
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| 650 |
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7 |
|a Arthropod Proteins
|2 NLM
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| 650 |
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7 |
|a Dermatophagoides farinae antigen f 2
|2 NLM
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| 650 |
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7 |
|a Disulfides
|2 NLM
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| 650 |
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7 |
|a Seed Storage Proteins
|2 NLM
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| 650 |
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|a Immunoglobulin E
|2 NLM
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| 650 |
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|a 37341-29-0
|2 NLM
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| 700 |
1 |
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|a Hirose, Sakiko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Suzuki, Kazuya
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hiroi, Takachika
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Takaiwa, Fumio
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 63(2012), 8 vom: 29. Mai, Seite 2947-59
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:63
|g year:2012
|g number:8
|g day:29
|g month:05
|g pages:2947-59
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|u http://dx.doi.org/10.1093/jxb/ers006
|3 Volltext
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|h 2947-59
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