Evaluation of DNA isolation procedures for detecting and quantifying environmental Legionella by real-time quantitative PCR

Six methods, QiAamp DNA Mini Kit (Q), Q with Sepharose 4B gel column (Q/G), Q with low melting point agarose (Q/L), freeze-thaw/phenol-chloroform lysis (FT-PC), FT-PC/G, and FT-PC/L, were evaluated for their ability to isolate DNA of sufficient quality to quantify Legionella using qPCR. Samples of m...

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Publié dans:	Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 65(2012), 6 vom: 21., Seite 989-97
Auteur principal:	Chen, N-T (Auteur)
Autres auteurs:	Chang, C-W, Wu, J-D
Format:	Article en ligne
Langue:	English
Publié:	2012
Accès à la collection:	Water science and technology : a journal of the International Association on Water Pollution Research
Sujets:	Journal Article Research Support, Non-U.S. Gov't DNA, Bacterial