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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ers009
|2 doi
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|a pubmed24n0718.xml
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|a (DE-627)NLM215524810
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|a (NLM)22345636
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a Zheng, Shu-Xiao
|e verfasserin
|4 aut
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|a The gene encoding Arabidopsis acyl-CoA-binding protein 3 is pathogen inducible and subject to circadian regulation
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 07.09.2012
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|a Date Revised 09.03.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a In Arabidopsis thaliana, acyl-CoA-binding protein 3 ( ACBP3), one of six ACBPs, is unique in terms of the C-terminal location of its acyl-CoA-binding domain. It promotes autophagy-mediated leaf senescence and confers resistance to Pseudomonas syringae pv. tomato DC3000. To understand the regulation of ACBP3, a 1.7 kb 5'-flanking region of ACBP3 and its deletion derivatives were characterized using β-glucuronidase (GUS) fusions. A 374 bp minimal fragment (-151/+223) could drive GUS expression while a 1698 bp fragment (-1475/+223) conferred maximal activity. Further, histochemical analysis on transgenic Arabidopsis harbouring the largest (1698 bp) ACBP3pro::GUS fusion displayed ubiquitous expression in floral organs and vegetative tissues (vascular bundles of leaves and stems), consistent with previous results showing that extracellularly localized ACBP3 functions in plant defence. A 160 bp region (-434/-274) induced expression in extended darkness and caused down-regulation in extended light. Electrophoretic mobility shift assay (EMSA) and DNase I footprinting assay showed that the DNA-binding with one finger box (Dof-box, -341/-338) interacted specifically with leaf nuclear proteins from dark-treated Arabidopsis, while GT-1 (-406/-401) binds both dark- and light-treated Arabidopsis, suggesting that Dof and GT-1 motifs are required to mediate circadian regulation of ACBP3. Moreover, GUS staining and fluorometric measurements revealed that a 109 bp region (-543/-434) was responsive to phytohormones and pathogens. An S-box of AT-rich sequence (-516/-512) was identified to bind nuclear proteins from pathogen-infected Arabidopsis leaves, providing the basis for pathogen-inducible regulation of ACBP3 expression. Thus, three cis-responsive elements (Dof, GT-1, and the S-box) in the 5'-flanking region of ACBP3 are proven functional in the regulation of ACBP3
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a ACBP3 protein, Arabidopsis
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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|a Carrier Proteins
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|a Plant Growth Regulators
|2 NLM
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|a Recombinant Fusion Proteins
|2 NLM
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|a Deoxyribonuclease I
|2 NLM
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| 650 |
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|a EC 3.1.21.1
|2 NLM
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|a Glucuronidase
|2 NLM
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| 650 |
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|a EC 3.2.1.31
|2 NLM
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| 700 |
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|a Xiao, Shi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chye, Mee-Len
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 63(2012), 8 vom: 15. Mai, Seite 2985-3000
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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|g volume:63
|g year:2012
|g number:8
|g day:15
|g month:05
|g pages:2985-3000
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|u http://dx.doi.org/10.1093/jxb/ers009
|3 Volltext
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