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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2011.11.012
|2 doi
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|a pubmed24n0718.xml
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|a (DE-627)NLM215335651
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|a (NLM)22325889
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Zhang, Zhixing
|e verfasserin
|4 aut
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| 245 |
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|a Proteomic and phosphoproteomic determination of ABA's effects on grain-filling of Oryza sativa L. inferior spikelets
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| 264 |
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 11.04.2013
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
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|a Cultivars of rice (Oryza sativa L.), especially the large-spikelet-type, often fail to achieve the high yield potential due to poor grain-filling of their inferior (late-flowering) spikelets. The superior (early-flowering) spikelets normally contain more abscisic acid (ABA) than the inferior spikelets. It was speculated that ABA might play a pivotal role in the grain-filling of inferior spikelets. To understand the molecular regulation involved in this process, we employed the 2-D gel-based comparative proteomic and phosphoproteomic analyses to search for differentially expressed proteins in the inferior spikelets under exogenous ABA treatment. A total of 111 significantly differential proteins and 31 phosphoproteins were found in the inferior spikelets after treatment. Among them, 100 proteins and 23 phosphoproteins were identified by using MALDI-TOF/TOF MS. In addition, the gene expression patterns of the inferior spikelets were confirmed with RT-PCR. These differentially expressed proteins are active in defense response, carbohydrate, protein, amino acid, energy and secondary metabolisms, as well as cell development and photosynthesis. The results suggest that the grain-filling of rice inferior spikelets is regulated by ABA through some proteins and phosphoproteins participating in carbon, nitrogen and energy metabolisms
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Plant Growth Regulators
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a RNA, Plant
|2 NLM
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| 650 |
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|a Abscisic Acid
|2 NLM
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| 650 |
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7 |
|a 72S9A8J5GW
|2 NLM
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| 700 |
1 |
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|a Chen, Jun
|e verfasserin
|4 aut
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| 700 |
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|a Lin, Shisheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Zhong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Ronghuai
|e verfasserin
|4 aut
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| 700 |
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|a Fang, Changxun
|e verfasserin
|4 aut
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| 700 |
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|a Chen, Hongfei
|e verfasserin
|4 aut
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| 700 |
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|a Lin, Wenxiong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 185-186(2012) vom: 10. Apr., Seite 259-73
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:185-186
|g year:2012
|g day:10
|g month:04
|g pages:259-73
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|u http://dx.doi.org/10.1016/j.plantsci.2011.11.012
|3 Volltext
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|d 185-186
|j 2012
|b 10
|c 04
|h 259-73
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