Oral DNA vaccination of rainbow trout, Oncorhynchus mykiss (Walbaum), against infectious haematopoietic necrosis virus using PLGA [Poly(D,L-Lactic-Co-Glycolic Acid)] nanoparticles
© 2012 Blackwell Publishing Ltd.
| Publié dans: | Journal of fish diseases. - 1998. - 35(2012), 3 vom: 27. März, Seite 203-14 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2012
|
| Accès à la collection: | Journal of fish diseases |
| Sujets: | Journal Article Research Support, Non-U.S. Gov't Antibodies, Neutralizing Antibodies, Viral Vaccines, DNA Viral Proteins Viral Vaccines Polylactic Acid-Polyglycolic Acid Copolymer 1SIA8062RS Polyglycolic Acid plus... |
| Résumé: | © 2012 Blackwell Publishing Ltd. A DNA vaccine against infectious haematopoietic necrosis virus (IHNV) is effective at protecting rainbow trout, Oncorhynchus mykiss, against disease, but intramuscular injection is required and makes the vaccine impractical for use in the freshwater rainbow trout farming industry. Poly (D,L-lactic-co-glycolic acid) (PLGA) is a U.S. Food and Drug Administration (FDA) approved polymer that can be used to deliver DNA vaccines. We evaluated the in vivo absorption of PLGA nanoparticles containing coumarin-6 when added to a fish food pellet. We demonstrated that rainbow trout will eat PLGA nanoparticle coated feed and that these nanoparticles can be detected in the epithelial cells of the lower intestine within 96 h after feeding. We also detected low levels of gene expression and anti-IHNV neutralizing antibodies when fish were fed or intubated with PLGA nanoparticles containing IHNV G gene plasmid. A virus challenge evaluation suggested a slight increase in survival at 6 weeks post-vaccination in fish that received a high dose of the oral vaccine, but there was no difference when additional fish were challenged at 10 weeks post-vaccination. The results of this study suggest that it is possible to induce an immune response using an orally delivered DNA vaccine, but the current system needs improvement |
|---|---|
| Description: | Date Completed 11.05.2012 Date Revised 30.09.2020 published: Print Citation Status MEDLINE |
| ISSN: | 1365-2761 |
| DOI: | 10.1111/j.1365-2761.2011.01338.x |