Length and GC-biases during sequencing library amplification : a comparison of various polymerase-buffer systems with ancient and modern DNA sequencing libraries
High-throughput sequencing technologies frequently necessitate the use of PCR for sequencing library amplification. PCR is a sometimes enigmatic process and is known to introduce biases. Here we perform a simple amplification-sequencing assay using 10 commercially available polymerase-buffer systems...
| Veröffentlicht in: | BioTechniques. - 1993. - 52(2012), 2 vom: 06. Feb., Seite 87-94 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
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2012
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Comparative Study Journal Article Research Support, Non-U.S. Gov't Buffers DNA-Directed DNA Polymerase EC 2.7.7.7 |
| Online verfügbar |
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