Identification and possible role of a MYB transcription factor from saffron (Crocus sativus)

Identification and possible role of a MYB transcription factor from saffron (Crocus sativus)

Copyright © 2012 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 169(2012), 5 vom: 15. März, Seite 509-15
1. Verfasser: Gómez-Gómez, Lourdes (VerfasserIn)
Weitere Verfasser: Trapero-Mozos, Almudena, Gómez, Maria Dolores, Rubio-Moraga, Angela, Ahrazem, Oussama
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Transcription Factors
Beschreibung
Zusammenfassung:Copyright © 2012 Elsevier GmbH. All rights reserved.
The MYB family is the most abundant group of transcription factors described for plants. Plant MYB genes have been shown to be involved in the regulation of many aspects of plant development. No MYB genes are described for saffron, the dried stigma of Crocus sativus, utilized as a colorant for foodstuffs. In this study, we used RACE-PCR to isolate a full length cDNA of 894bp with a 591bp open reading frame, encoding a putative CsMYB1 from C. sativus. Comparison between gDNA and cDNA revealed no introns. Homology studies indicated that the deduced amino acid sequence is similar to members of the R2R3 MYB subfamily. Expression analysis showed the presence of high transcript levels in stigma tissue and low levels in tepals, whereas no signal was detected in either anthers or leaves. The RT-PCR analysis revealed that CsMYB1 expression is developmentally regulated during stigma development. Furthermore, expression analysis in stigmas from different Crocus species showed a correlation with stigma morphology. No transcripts were found in stigma tissues of Crocus species characterized by branched stigma morphology. Taken together, these results suggest that CsMYB1 may be involved in the regulation of stigma morphology in Crocus
Beschreibung:Date Completed 15.08.2012
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1618-1328
DOI:10.1016/j.jplph.2011.11.021