Musa paradisica RCI complements AtRCI and confers Na+ tolerance and K+ sensitivity in Arabidopsis

Bibliographische Detailangaben
Veröffentlicht in:	Plant science : an international journal of experimental plant biology. - 1985. - 184(2012) vom: 29. März, Seite 102-11
1. Verfasser:	Liu, Bing (VerfasserIn)
Weitere Verfasser:	Feng, Dongru, Zhang, Bipei, Mu, Peiqiang, Zhang, Yang, He, Yanming, Qi, Kangbiao, Wang, Jinfa, Wang, Hongbin
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2012
Zugriff auf das übergeordnete Werk:	Plant science : an international journal of experimental plant biology
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't PMP3 protein, S cerevisiae Plant Proteins Proteolipids Saccharomyces cerevisiae Proteins Sodium Chloride 451W47IQ8X Potassium Chloride 660YQ98I10 mehr... Sodium 9NEZ333N27 Proton-Translocating ATPases EC 3.6.3.14 Potassium RWP5GA015D

Beschreibung
Zusammenfassung:	Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. The mechanisms involved in Na⁺/K⁺ uptake and extrusion are important in plant salt tolerance. In this study, we investigated the physiological role of a plasma membrane (PM)-localized protein, MpRCI, from plantain in transgenic Arabidopsis under NaCl and KCl stress and determined its effect on PM fluidity and H⁺-ATPase activity. The MpRCI gene exhibited high homology to the AtRCI2 gene family in Arabidopsis and was therefore able to complement for loss of the yeast AtRCI2-related PMP3 gene. Results of phenotypic espial and atomic emission spectrophotometer (AES) assays indicated that MpRCI overexpression in the AtRCI2A knockout mutant with reduced shoot Na⁺ and increased K⁺ exhibited increased Na⁺-tolerance and K⁺-sensitivity under NaCl or KCl treatments, respectively. Furthermore, comparisons of PM fluidity and H⁺-ATPase activity in shoots, with expression or absence of MpRCI/AtRCI2A expression under NaCl or KCl stress, showed MpRCI maintained PM fluidity and H⁺-ATPase activity under stress conditions. Results suggest that MpRCI plays an essential role in Na⁺/K⁺ flux in plant cells
Beschreibung:	Date Completed 16.05.2012 Date Revised 30.09.2020 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2259
DOI:	10.1016/j.plantsci.2011.12.004