Identification of an E-box motif responsible for the expression of jasmonic acid-induced chitinase gene OsChia4a in rice

Copyright © 2011 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 169(2012), 6 vom: 15. Apr., Seite 621-7
1. Verfasser: Miyamoto, Koji (VerfasserIn)
Weitere Verfasser: Shimizu, Takafumi, Lin, Fengqiu, Sainsbury, Frank, Thuenemann, Eva, Lomonossoff, George, Nojiri, Hideaki, Yamane, Hisakazu, Okada, Kazunori
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Antifungal Agents Basic Helix-Loop-Helix Transcription Factors Cyclopentanes Oxylipins jasmonic acid 6RI5N05OWW Chitinases EC 3.2.1.14
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245 1 0 |a Identification of an E-box motif responsible for the expression of jasmonic acid-induced chitinase gene OsChia4a in rice 
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520 |a The plant hormone jasmonic acid (JA) is known to be involved in multiple defence responses against pathogens, which include the production of pathogenesis-related (PR) proteins. In order to investigate the induction mechanism of the rice defence responses by JA, we performed transcriptome analyses and focused on a chitinase gene, OsChia4a, which was identified to be one of the highest JA-inductive genes. The recombinant protein of His-tagged OsChia4a exhibited an inhibitory effect against the spore germination and hyphal growth of Magnaporthe oryzae. The promoter analysis of OsChia4a revealed that the region from -515 bp to -265 bp upstream of the ATG translation initiation site was required for the responsiveness to JA. A subsequent mutation analysis indicated that an E-box (CANNTG) in this region act as a JA-responsive cis element. These results imply that a basic helix-loop-helix transcription factor is likely to be involved in the regulation of the OsChia4a expression in a JA-dependent manner 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Antifungal Agents  |2 NLM 
650 7 |a Basic Helix-Loop-Helix Transcription Factors  |2 NLM 
650 7 |a Cyclopentanes  |2 NLM 
650 7 |a Oxylipins  |2 NLM 
650 7 |a jasmonic acid  |2 NLM 
650 7 |a 6RI5N05OWW  |2 NLM 
650 7 |a Chitinases  |2 NLM 
650 7 |a EC 3.2.1.14  |2 NLM 
700 1 |a Shimizu, Takafumi  |e verfasserin  |4 aut 
700 1 |a Lin, Fengqiu  |e verfasserin  |4 aut 
700 1 |a Sainsbury, Frank  |e verfasserin  |4 aut 
700 1 |a Thuenemann, Eva  |e verfasserin  |4 aut 
700 1 |a Lomonossoff, George  |e verfasserin  |4 aut 
700 1 |a Nojiri, Hideaki  |e verfasserin  |4 aut 
700 1 |a Yamane, Hisakazu  |e verfasserin  |4 aut 
700 1 |a Okada, Kazunori  |e verfasserin  |4 aut 
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