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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1021/la204089u
|2 doi
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|a pubmed24n0715.xml
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|a (DE-627)NLM21470680X
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|a (NLM)22260723
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Chen, Qibin
|e verfasserin
|4 aut
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|a Structure of the complex monolayer of gemini surfactant and DNA at the air/water interface
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 30.07.2012
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The properties of the complex monolayers composed of cationic gemini surfactants, [C(18)H(37)(CH(3))(2)N(+)-(CH(2))(s)-N(+)(CH(3))(2)C(18)H(37)],2Br(-) (18-s-18 with s = 3, 4, 6, 8, 10 and 12), and ds-DNA or ss-DNA at the air/water interface were in situ studied by the surface pressure-area per molecule (π-A) isotherm measurement and the infrared reflection absorption spectroscopy (IRRAS). The corresponding Langmuir-Blodgett (LB) films were also investigated by the atomic force microscopy (AFM), the Fourier transform infrared spectroscopy (FT-IR), and the circular dichroism spectroscopy (CD). The π-A isotherms and AFM images reveal that the spacer of gemini surfactant has a significant effect on the surface properties of the complex monolayers. As s ≤ 6, the gemini/ds-DNA complex monolayers can both laterally and normally aggregate to form fibril structures with heights of 2.0-7.0 nm and widths of from several tens to ~300 nm. As s > 6, they can laterally condense to form the platform structure with about 1.4 nm height. Nevertheless, FT-IR, IRRAS, and CD spectra, as well as AFM images, suggest that DNA retains its double-stranded character when complexed. This is very important and meaningful for gene therapy because it is crucial to maintain the extracellular genes undamaged to obtain a high transfection efficiency. In addition, when s ≤ 6, the gemini/ds-DNA complex monolayers can experience a transition of DNA molecule from the double-stranded helical structure to a typical ψ-phase with a supramolecular chiral order
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cations
|2 NLM
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|a Surface-Active Agents
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|a Water
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|a 059QF0KO0R
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a Kang, Xueli
|e verfasserin
|4 aut
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| 700 |
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|a Li, Rong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Du, Xuezhong
|e verfasserin
|4 aut
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|a Shang, Yazhuo
|e verfasserin
|4 aut
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|a Liu, Honglai
|e verfasserin
|4 aut
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|a Hu, Ying
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 28(2012), 7 vom: 21. Feb., Seite 3429-38
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:28
|g year:2012
|g number:7
|g day:21
|g month:02
|g pages:3429-38
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|u http://dx.doi.org/10.1021/la204089u
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