Phenotyping transgenic wheat for drought resistance

Realistic experimental protocols to screen for drought adaptation in controlled conditions are crucial if high throughput phenotyping is to be used for the identification of high performance lines, and is especially important in the evaluation of transgenes where stringent biosecurity measures restr...

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Veröffentlicht in:Journal of experimental botany. - 1985. - 63(2012), 5 vom: 16. März, Seite 1799-808
1. Verfasser: Saint Pierre, Carolina (VerfasserIn)
Weitere Verfasser: Crossa, José L, Bonnett, David, Yamaguchi-Shinozaki, Kazuko, Reynolds, Matthew P
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Arabidopsis Proteins DREB1A protein, Arabidopsis Transcription Factors Water 059QF0KO0R
Beschreibung
Zusammenfassung:Realistic experimental protocols to screen for drought adaptation in controlled conditions are crucial if high throughput phenotyping is to be used for the identification of high performance lines, and is especially important in the evaluation of transgenes where stringent biosecurity measures restrict the frequency of open field trials. Transgenic DREB1A-wheat events were selected under greenhouse conditions by evaluating survival and recovery under severe drought (SURV) as well as for water use efficiency (WUE). Greenhouse experiments confirmed the advantages of transgenic events in recovery after severe water stress. Under field conditions, the group of transgenic lines did not generally outperform the controls in terms of grain yield under water deficit. However, the events selected for WUE were identified as lines that combine an acceptable yield-even higher yield (WUE-11) under well irrigated conditions-and stable performance across the different environments generated by the experimental treatments
Beschreibung:Date Completed 04.06.2014
Date Revised 18.03.2022
published: Print-Electronic
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/err385