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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1021/la2038075
|2 doi
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|a pubmed25n0714.xml
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|a eng
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|a Kashiwada, Ayumi
|e verfasserin
|4 aut
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|a Design, construction, and characterization of high-performance membrane fusion devices with target-selectivity
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 23.05.2012
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|a Date Revised 15.11.2012
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2011 American Chemical Society
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|a Membrane fusion proteins such as the hemagglutinin glycoprotein have target recognition and fusion accelerative domains, where some synergistically working elements are essential for target-selective and highly effective native membrane fusion systems. In this work, novel membrane fusion devices bearing such domains were designed and constructed. We selected a phenylboronic acid derivative as a recognition domain for a sugar-like target and a transmembrane-peptide (Leu-Ala sequence) domain interacting with the target membrane, forming a stable hydrophobic α-helix and accelerating the fusion process. Artificial membrane fusion behavior between the synthetic devices in which pilot and target liposomes were incorporated was characterized by lipid-mixing and inner-leaflet lipid-mixing assays. Consequently, the devices bearing both the recognition and transmembrane domains brought about a remarkable increase in the initial rate for the membrane fusion compared with the devices containing the recognition domain alone. In addition, a weakly acidic pH-responsive device was also constructed by replacing three Leu residues in the transmembrane-peptide domain by Glu residues. The presence of Glu residues made the acidic pH-dependent hydrophobic α-helix formation possible as expected. The target-selective liposome-liposome fusion was accelerated in a weakly acidic pH range when the Glu-substituted device was incorporated in pilot liposomes. The use of this pH-responsive device seems to be a potential strategy for novel applications in a liposome-based delivery system
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|a Journal Article
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|a Boronic Acids
|2 NLM
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|a Liposomes
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|a Peptides
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|a benzeneboronic acid
|2 NLM
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|a L12H7B02G5
|2 NLM
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| 700 |
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|a Yamane, Iori
|e verfasserin
|4 aut
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|a Tsuboi, Mana
|e verfasserin
|4 aut
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| 700 |
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|a Ando, Shun
|e verfasserin
|4 aut
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| 700 |
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|a Matsuda, Kiyomi
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 28(2012), 4 vom: 31. Jan., Seite 2299-305
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:28
|g year:2012
|g number:4
|g day:31
|g month:01
|g pages:2299-305
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|u http://dx.doi.org/10.1021/la2038075
|3 Volltext
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