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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2011.10.004
|2 doi
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|a pubmed24n0712.xml
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|a (DE-627)NLM213700913
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|a (NLM)22153235
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|a DE-627
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|c DE-627
|e rakwb
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|a eng
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|a Flachowsky, Henryk
|e verfasserin
|4 aut
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|a Silencing of flavanone-3-hydroxylase in apple (Malus × domestica Borkh.) leads to accumulation of flavanones, but not to reduced fire blight susceptibility
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 16.04.2012
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2011 Elsevier Masson SAS. All rights reserved.
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|a Transgenic antisense flavanone-3-hydroxylase apple plants were produced to mimic the effect of the agrochemical prohexadione-Ca on apple leaves. This enzyme inhibitor for 2-oxoglutarate dependent dioxygenases is used as a growth retardant and for control of secondary fire blight of leaves. Like using the agent, silencing of flavanone-3-hydroxylase leads to an accumulation of flavanones in leaves, but in contrast not to the formation of 3-deoxyflavonoids. In prohexadione-Ca treated leaves the 3-deoxyflavonoid luteoforol is formed from accumulating flavanones, acting as an antimicrobial compound against the fire blight pathogen Erwinia amylovora. Seemingly, the silencing of just one of the 2-oxoglutarate dependent dioxygenases (in apple also flavonol synthase and anthocyanidin synthase take part downstream in the pathway) does not provide a sufficiently high ratio of flavanones to dihydroflavonols. This seems to be needed to let the dihydroflavonol-4-reductase/flavanone-4-reductase enzyme reduce flavanones to luteoforol, and to let this be reduced by the leucoanthocyanidin-4-reductase/3-deoxyleucoanthocyanidin-4-reductase, each acting with their respective weak secondary activities. Accordingly, also the intended inducible resistance to fire blight by prohexadione-Ca is not observed with the antisense flavanone-3-hydroxylase apple plants. On the other hand, for most transgenic lines with strong flavanone-4-reductase down-regulation, up-regulation of gene expression for the other flavonoid genes was found. This provides further evidence for the feedback regulation of flavonoid gene expression having been previously reported for the prohexadione-Ca inhibited apple plants
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Anti-Bacterial Agents
|2 NLM
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|a Benzopyrans
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|a Culture Media
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|a Enzyme Inhibitors
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|a Flavanones
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|a Ketoglutaric Acids
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|a Plant Proteins
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|a prohexadione-Ca
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|a luteoforol
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|a 24897-98-1
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|a Mixed Function Oxygenases
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|a EC 1.-
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|a Alcohol Oxidoreductases
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|a EC 1.1.-
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|a dihydroflavanol 4-reductase
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|a EC 1.1.1.-
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|a flavanone 3-dioxygenase
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|a EC 1.14.11.9
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|a Halbwirth, Heidi
|e verfasserin
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|a Treutter, Dieter
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|a Richter, Klaus
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|a Hanke, Magda-Viola
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|a Szankowski, Iris
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|a Gosch, Christian
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|a Stich, Karl
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|a Fischer, Thilo C
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 51(2012) vom: 01. Feb., Seite 18-25
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:51
|g year:2012
|g day:01
|g month:02
|g pages:18-25
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|u http://dx.doi.org/10.1016/j.plaphy.2011.10.004
|3 Volltext
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|d 51
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|h 18-25
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