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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1021/la203770j
|2 doi
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|a pubmed25n0712.xml
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|a eng
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1 |
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|a Ningsih, Zubaidah
|e verfasserin
|4 aut
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|a Slow insertion kinetics during interaction of a model antimicrobial peptide with unilamellar phospholipid vesicles
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 23.05.2012
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|a Date Revised 31.01.2012
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2011 American Chemical Society
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|a The mechanism of interaction between a model antimicrobial peptide and phospholipid unilamellar vesicle membranes was studied using fluorescence spectroscopy, fluorescence lifetime measurements, and light scattering. The peptide, a mellitin mutant, was labeled at position K14 with the polarity-sensitive probe AlexaFluor 430. The kinetics of the interaction of this derivative with various concentrations of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) vesicles was examined. Our work unveiled two novel aspects of peptide-lipid interactions. First, the AB plot or phasor analysis of the fluorescence lifetime studies revealed at least three different peptide states, the population of which depended on the lipid to peptide (L:P) concentration ratio. Second, complex fluorescence kinetics were observed over extended time-scales from 30 s to 2 h. The extended kinetics was only observed at particular lipid concentrations (L:P ratios 20:1 and 10:1) and not at others (30, 40, 50 and 100:1 L:P ratio). Analysis of the complex kinetics revealed several intermediates. We assign these to distinct states of the peptide formed during helix insertion into the vesicle membrane that are intermediate to lytic pore formation
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|a Journal Article
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|a Fluorescent Dyes
|2 NLM
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|a Unilamellar Liposomes
|2 NLM
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|a Melitten
|2 NLM
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|a 20449-79-0
|2 NLM
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|a 1,2-Dipalmitoylphosphatidylcholine
|2 NLM
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|a 2644-64-6
|2 NLM
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|a Hossain, Mohammed Akhter
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wade, John D
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Clayton, Andrew H A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gee, Michelle L
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 28(2012), 4 vom: 31. Jan., Seite 2217-24
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|g volume:28
|g year:2012
|g number:4
|g day:31
|g month:01
|g pages:2217-24
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|u http://dx.doi.org/10.1021/la203770j
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