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231224s2012 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la203954x
|2 doi
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|a pubmed24n0711.xml
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|a (NLM)22103891
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Ge, Lin
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Investigation of the interaction between a bivalent aptamer and thrombin by AFM
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 25.04.2012
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|a Date Revised 10.01.2012
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Aptamers are a new class of molecular probes for protein recognition, detection, and inhibition. Multivalent aptamer-protein binding through aptamer assembly has been currently developed as an effective way to achieve higher protein affinity and selectivity. In this study, the specific interaction between bivalent aptamer Bi-8S and thrombin has been measured directly and quantitatively by atomic force microscopy to investigate the unbinding dynamics and dissociation energy landscape of the multivalent interaction. Bivalent aptamer Bi-8S contains thrombin's two aptamers, 15apt and 27apt, which are linked by eight spacer phosphoramidites. The results revealed the sequential dissociation of the two aptamers. Moreover, the dynamic force spectroscopy data revealed that the 27apt's binding to the thrombin remains largely unaffected by the eight-spacer phosphoramidites within Bi-8S. In contrast, the eight-spacer phosphoramidites stabilized the 15apt-thrombin binding
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| 650 |
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|a Journal Article
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| 650 |
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Aptamers, Nucleotide
|2 NLM
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| 650 |
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|a Thrombin
|2 NLM
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| 650 |
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|a EC 3.4.21.5
|2 NLM
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| 700 |
1 |
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|a Jin, Gang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Xiaohong
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 28(2012), 1 vom: 10. Jan., Seite 707-13
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:28
|g year:2012
|g number:1
|g day:10
|g month:01
|g pages:707-13
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|u http://dx.doi.org/10.1021/la203954x
|3 Volltext
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