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231224s2011 xx ||||| 00| ||chi c |
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|a pubmed25n0710.xml
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|a (DE-627)NLM213133423
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|a (NLM)22093310
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a chi
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| 100 |
1 |
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|a Wu, Yan
|e verfasserin
|4 aut
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|a The role of heme oxygenase 1 in hydrogen peroxide induced apoptosis and mitochondrial trans membrane potential change in rat primary type II alveolar epithelium cells
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 14.06.2012
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|a Date Revised 25.11.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To understand the role of heme oxygenase-1 (HO-1) in hydrogen peroxide [H(2)O(2)] induced apoptosis and mitochondrial trans-membrane potential (MTMP) change in primary alveolar epithelial cell type II(AEC II)
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|a METHODS: Primary AEC II collected from healthy Sprague Dawley (SD) rats were cultured for 24 hours, then divided into four groups to be treated with: (1) saline; (2) H(2)O(2) (0.5 mmol/L); (3) H(2)O(2) +HO-1 (0.2 mmol/L); (4) H(2)O(2) +zinc original porphyrin IX (HO-1 inhibitor, 20 μmol/L). The morphology of cells in the cultures was examined by fluorescent microscopy 2.5 hours later, and the number of apoptotic cells / the MTMP determined by flow-cytometry 0.5, 1.0, 1.5, 2.0 and 2.5 hours later
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|a RESULTS: Large number of cells in with green (early apoptotic) or red (later apoptotic) fluorescence were observed by microscope in cultures treated with H(2)O(2) , and H(2)O(2) + HO-1 inhibitor, but such cells were obviously fewer in HO-1 treated cultures. Compared with saline treated cells, H(2)O(2) treated cells had significantly higher apoptosis rate, that increased with time, reaching peak value 2.5 hours into the treatment [0.5 hour: (30.27 ± 0.74)% vs. (3.76 ± 0.81)%, 2.5 hours: (40.46 ± 0.91)% vs. (22.74 ± 0.60)%, both P < 0.05], while the rate of MTMP depolarization was significantly lower (0.99 ± 0.21 vs. 1.91 ± 0.16, P < 0.05) in these cells. Compared with H(2)O(2) treated cells, the apoptosis rate in HO-1 treated cells was significantly lower [0.5 hour: (5.99 ± 0.60)% vs. (30.27 ± 0.74)%, 2.5 hours: (22.69 ± 1.69)% vs. (40.46 ± 0.91)%, both P < 0.05], and their rate of MTMP depolarization higher (2.02 ± 0.12 vs. 0.99 ± 0.21, P < 0.05). Compared with HO-1 treated cells, HO-1 inhibitor treated cells had significantly higher apoptosis rate which reached peak value 2.5 hours into the treatment [0.5 hour: (30.73 ± 1.08)% vs. (5.99 ± 0.60)%, 2.5 hours: (41.38 ± 0.57)% vs. (22.69 ± 1.69)%, both P < 0.05], while rate of MTMP depolarization in these cells was significantly lower (0.98 ± 0.09 vs. 2.02 ± 0.12, P < 0.05)
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|a CONCLUSION: HO-1 could maintain the integrity of AEC II and stabilize their mitochondria membrane potential, protecting the cells from H(2)O(2) induced damage
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Hydrogen Peroxide
|2 NLM
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| 650 |
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|a BBX060AN9V
|2 NLM
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| 650 |
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|a Heme Oxygenase-1
|2 NLM
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| 650 |
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|a EC 1.14.14.18
|2 NLM
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| 700 |
1 |
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|a Chen, Miao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Shuang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Hong-min
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qian, Ming-jiang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Yu-hui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fu, Xiao-yun
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 23(2011), 11 vom: 17. Nov., Seite 658-60
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
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|g volume:23
|g year:2011
|g number:11
|g day:17
|g month:11
|g pages:658-60
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|a GBV_USEFLAG_A
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|a SYSFLAG_A
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 23
|j 2011
|e 11
|b 17
|c 11
|h 658-60
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