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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2011.08.013
|2 doi
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|a pubmed24n0707.xml
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|a (DE-627)NLM212238086
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|a (NLM)22000056
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Hadi, Fazal
|e verfasserin
|4 aut
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|a Identification and expression analysis of CBF/DREB1 and COR15 genes in mutants of Brassica oleracea var. botrytis with enhanced proline production and frost resistance
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 12.03.2014
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2011 Elsevier Masson SAS. All rights reserved.
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|a Frost resistant mutants of Brassica oleracea var. botrytis were investigated for the presence of CBF/DREB1 and COR15a gene products and induced frost resistance. Total RNA of clones was isolated after 3 h, 6 h, 24 h and 14 d acclimation at 4 °C and proteins and free proline were isolated after 14 d acclimation. cDNA was produced using RT-PCR and the first CBF gene in B. oleracea detected and did quantify. Through SDS-PAGE and Western blotting, the COR15a protein was detected for the first time in B. oleracea. The results confirmed the first report of the presence of BoCBF/DREB1 in B. oleracea and this only appeared under cold acclimation. The sequence analysis of predicted amino acids revealed a very high homology (90%) with CBF sequences of other Brassica species (BnCBF5/DREB1, BrDREB1 and BjDREB1B) and homology reduced to 67% when compared to plants other than Brassicas. BoCBF/DREB1 transcript levels increased up to 24 h acclimation and then declined. Some mutants showed BoCBF/DREB1 expression at 3 h while others only after 6 h and 24 h acclimation. The genotypes showed positive significant correlation between BoCBF/DREB1 expression and frost resistance (R(2) = 0.9343). The proline level under acclimation increased about 8 fold and demonstrated positive and significant correlation with BoCBF/DREB1 expression. Proline also showed positive and significant correlation with frost resistance under cold acclimation but very not under non-acclimation. All clones were positive for COR15a protein after 14 d cold acclimation and expression correlated with frost resistance. Under non-acclimation COR15a was constitutively expressed in 3 mutants
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA, Complementary
|2 NLM
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| 650 |
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|a DNA, Plant
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a RNA, Plant
|2 NLM
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| 650 |
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|a Transcription Factors
|2 NLM
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| 650 |
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|a Proline
|2 NLM
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| 650 |
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|a 9DLQ4CIU6V
|2 NLM
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| 700 |
1 |
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|a Gilpin, Martyn
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fuller, Michael P
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 49(2011), 11 vom: 14. Nov., Seite 1323-32
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
1 |
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|g volume:49
|g year:2011
|g number:11
|g day:14
|g month:11
|g pages:1323-32
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|u http://dx.doi.org/10.1016/j.plaphy.2011.08.013
|3 Volltext
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|a AR
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|d 49
|j 2011
|e 11
|b 14
|c 11
|h 1323-32
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