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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2011.03.004
|2 doi
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|a pubmed25n0706.xml
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|a (DE-627)NLM211856487
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|a (NLM)21958704
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Omar, Samar A
|e verfasserin
|4 aut
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| 245 |
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|a Identification and expression analysis of two small heat shock protein cDNAs from developing seeds of biodiesel feedstock plant Jatropha curcas
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 23.01.2012
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|a Date Revised 15.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
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|a Plant small heat shock proteins (sHSPs) are known to be important for environmental stress tolerance and involved in various developmental processes. In this study, two full-length cDNAs encoding sHSPs, designated JcHSP-1 and JcHSP-2, were identified and characterized from developing seeds of a promising biodiesel feedstock plant Jatropha curcas by expressed sequence tag (EST) sequencing of embryo cDNA libraries and rapid amplification of cDNA ends (RACE). JcHSP-1 and JcHSP-2 contained open-reading frames encoding sHSPs of 219 and 157 amino acids, with predicted molecular weights of 24.42kDa and 18.02kDa, respectively. Sequence alignment indicated that both JcHSP-1 and JcHSP-2 shared high similarity with other plant sHSPs. Real-time quantitative RT-PCR analysis showed that the transcriptional level of both JcHSP-1 and JcHSP-2 increased along with natural dehydration process during seed development. A sharp increase of JcHSP-2 transcripts occurred in response to water content dropping from 42% in mature seeds to 12% in dry seeds. Western blot analysis revealed that the accumulation profile of two cross-reacting proteins, whose molecular weight corresponding to the calculated size of JcHSP-1 and JcHSP-2, respectively, was well consistent with the mRNA expression pattern of JcHSP-1 and JcHSP-2 in jatropha seeds during maturation and natural dehydration. These results indicated that both JcHSPs might play an important role in cell protection and seed development during maturation of J. curcas seeds
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Biofuels
|2 NLM
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| 650 |
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|a DNA, Complementary
|2 NLM
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| 650 |
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7 |
|a Heat-Shock Proteins, Small
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Fu, Qian-Tang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Mao-Sheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Gui-Juan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Song, Song-Quan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Elsheery, Nabil I
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Zeng Fu
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 181(2011), 6 vom: 06. Dez., Seite 632-7
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
1 |
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|g volume:181
|g year:2011
|g number:6
|g day:06
|g month:12
|g pages:632-7
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|u http://dx.doi.org/10.1016/j.plantsci.2011.03.004
|3 Volltext
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|d 181
|j 2011
|e 6
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|c 12
|h 632-7
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