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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la2032434
|2 doi
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|a pubmed24n1337.xml
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|a (DE-627)NLM211575747
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|a (NLM)21928859
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Ma, Yong
|e verfasserin
|4 aut
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| 245 |
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|a Azide-reactive liposome for chemoselective and biocompatible liposomal surface functionalization and glyco-liposomal microarray fabrication
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|c 2011
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 16.02.2012
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|a Date Revised 20.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Chemically selective liposomal surface functionalization and liposomal microarray fabrication using azide-reactive liposomes are described. First, liposome carrying PEG-triphenylphosphine was prepared for Staudinger ligation with azide-containing biotin, which was conducted in PBS buffer (pH 7.4) at room temperature without a catalyst. Then, immobilization and microarray fabrication of the biotinylated liposome onto a streptavidin-modified glass slide via the specific streptavidin/biotin interaction were investigated by comparing with directly formed biotin-liposome, which was prepared by the conventional liposome formulation of lipid-biotin with all other lipid components. Next, the covalent microarray fabrication of liposome carrying triphenylphosphine onto an azide-modified glass slide and its further glyco-modification with azide-containing carbohydrate were demonstrated for glyco-liposomal microarray fabrication via Staudinger ligation. Fluorescence imaging confirmed the successful immobilization and protein binding of the intact immobilized liposomes and arrayed glyco-liposomes. The azide-reactive liposome provides a facile strategy for membrane-mimetic glyco-array fabrication, which may find important biological and biomedical applications such as studying carbohydrate-protein interactions and toxin and antibody screening
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Azides
|2 NLM
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|a Carbohydrates
|2 NLM
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| 650 |
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|a Coated Materials, Biocompatible
|2 NLM
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| 650 |
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7 |
|a Liposomes
|2 NLM
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| 650 |
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7 |
|a Organophosphorus Compounds
|2 NLM
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| 650 |
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7 |
|a triphenylphosphine
|2 NLM
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| 650 |
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7 |
|a 26D26OA393
|2 NLM
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| 700 |
1 |
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|a Zhang, Hailong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Gruzdys, Valentinas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Xue-Long
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 21 vom: 01. Nov., Seite 13097-103
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:27
|g year:2011
|g number:21
|g day:01
|g month:11
|g pages:13097-103
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|u http://dx.doi.org/10.1021/la2032434
|3 Volltext
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|h 13097-103
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