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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1007/s10646-011-0772-4
|2 doi
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|a pubmed24n1253.xml
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|a (DE-627)NLM211397644
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|a (NLM)21909961
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Owen, Jennifer R
|e verfasserin
|4 aut
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| 245 |
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|a Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 24.04.2012
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|a Date Revised 09.01.2024
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|a published: Print-Electronic
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|a GENBANK: X98885, X98886
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|a Citation Status MEDLINE
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|a The macro-alga Fucus vesiculosus has a broad global and estuarine distribution and exhibits exceptional resistance to toxic metals, the molecular basis of which is poorly understood. To address this issue a cDNA library was constructed from an environmental isolate of F. vesiculosus growing in an area with chronic copper pollution. Characterisation of this library led to the identification of a cDNA encoding a protein known to be synthesised in response to toxicity, a full length 14-3-3 exhibiting a 71% identity to human/mouse epsilon isoform, 70-71% identity to yeast BMH1/2 and 95 and 71% identity to the Ectocarpus siliculosus 14-3-3 isoforms 1 and 2 respectively. Preliminary characterisation of the expression profile of the 14-3-3 indicated concentration- and time-dependent inductions on acute exposure of F. vesiculosus of copper (3-30 μg/l). Higher concentrations of copper (≥150 μg/l) did not elicit significant induction of the 14-3-3 gene compared with the control even though levels of both intracellular copper and the expression of a cytosolic metal chaperone, metallothionein, continued to rise. Analysis of gene expression within environmental isolates demonstrated up-regulation of the 14-3-3 gene associated with the known copper pollution gradient. Here we report for the first time, identification of a gene encoding a putative 14-3-3 protein in a multicellular alga and provide preliminary evidence to link the induction of this 14-3-3 gene to copper exposure in this alga. Interestingly, the threshold exposure profile may be associated with a decrease in the organism's ability to control copper influx so that it perceives copper as a toxic response
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a 14-3-3 Proteins
|2 NLM
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| 650 |
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|a DNA, Complementary
|2 NLM
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| 650 |
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|a Copper
|2 NLM
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| 650 |
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|a 789U1901C5
|2 NLM
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| 700 |
1 |
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|a Morris, Ceri A
|e verfasserin
|4 aut
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1 |
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|a Nicolaus, Beate
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Harwood, John L
|e verfasserin
|4 aut
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| 700 |
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|a Kille, Peter
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 21(2012), 1 vom: 15. Jan., Seite 124-38
|w (DE-627)NLM098212214
|x 1573-3017
|7 nnns
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| 773 |
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|g volume:21
|g year:2012
|g number:1
|g day:15
|g month:01
|g pages:124-38
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|u http://dx.doi.org/10.1007/s10646-011-0772-4
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