In-solution staining and arraying method for the immunofluorescence detection of γH2AX foci optimized for clinical applications
Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution bef...
Ausführliche Beschreibung
Bibliographische Detailangaben
Veröffentlicht in: | BioTechniques. - 1991. - 51(2011), 3 vom: 01. Sept., Seite 185-9
|
1. Verfasser: |
Johansson, Pegah
(VerfasserIn) |
Weitere Verfasser: |
Muslimovic, Aida,
Hultborn, Ragnar,
Fernström, Erik,
Hammarsten, Ola |
Format: | Online-Aufsatz
|
Sprache: | English |
Veröffentlicht: |
2011
|
Zugriff auf das übergeordnete Werk: | BioTechniques
|
Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Aminoglycosides
Enediynes
H2AX protein, human
Histones
calicheamicin T
142518-72-7 |