Fusion of lipid vesicles with planar lipid bilayers induced by a combination of peptides

Bibliographische Detailangaben
Veröffentlicht in:	Langmuir : the ACS journal of surfaces and colloids. - 1985. - 27(2011), 20 vom: 18. Okt., Seite 12515-20
1. Verfasser:	Inaba, Takehiko (VerfasserIn)
Weitere Verfasser:	Tatsu, Yoshiro, Morigaki, Kenichi
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2011
Zugriff auf das übergeordnete Werk:	Langmuir : the ACS journal of surfaces and colloids
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Fluorescent Dyes Lipid Bilayers Liposomes Peptides

Beschreibung
Zusammenfassung:	© 2011 American Chemical Society We studied the peptide-induced membrane fusion process between small unilamellar vesicles (SUVs) and supported planar bilayers (SPBs) with the aim of developing a method for incorporating membrane components into SPBs. As fusogenic peptides, two analogues of the N-terminal region of an influenza membrane fusion protein hemaggulutinin, anionic E5 and cationic K5, were synthesized, and the membrane fusion was investigated using SPB and SUVs composed of phosphatidylcholine from egg yolk (EggPC). We directly visualized the process of lipid transfer from SUVs to SPB by total internal reflection fluorescence (TIRF) microscopy. The transfer of fluorescent lipids was effectively induced only by the combination of two peptides. The TIRF microscopy observations of single SUV fusion events also revealed that lipid membranes from SUV could completely fuse into the SPB. However, the presence of single peptide (either E5 or K5) rather inhibited the lipid transfer, presumably due to the electrostatic repulsion between SUVs and SPB. The opposite effects induced by the peptides indicate the possibility for a designed application of two peptides as a means to control the membrane fusion spatially and temporally
Beschreibung:	Date Completed 14.02.2012 Date Revised 11.10.2011 published: Print-Electronic Citation Status MEDLINE
ISSN:	1520-5827
DOI:	10.1021/la2033548