Immobilization of horseradish peroxidase on silane-modified ceramics and their properties : potential for oily wastewater treatment

Horseradish peroxidase (HRP) is proved being effective in eliminating oil from aqueous solutions, but the elimination is expensive because free HRP can not be reused. In present work, HRP was successfully immobilized on cordierite porous ceramics support with a novel method of N-beta-aminoethyl-gamm...

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Publié dans:Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 63(2011), 8 vom: 07., Seite 1621-8
Auteur principal: Liu, W (Auteur)
Autres auteurs: Wang, W C, Li, H S, Zhou, X
Format: Article
Langue:English
Publié: 2011
Accès à la collection:Water science and technology : a journal of the International Association on Water Pollution Research
Sujets:Journal Article Enzymes, Immobilized Oils Silanes Water Pollutants, Chemical Horseradish Peroxidase EC 1.11.1.- Glutaral T3C89M417N
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520 |a Horseradish peroxidase (HRP) is proved being effective in eliminating oil from aqueous solutions, but the elimination is expensive because free HRP can not be reused. In present work, HRP was successfully immobilized on cordierite porous ceramics support with a novel method of N-beta-aminoethyl-gamma-aminopropyl-trimethoxysilane modification and glutaraldehyde activation. Under the optimized immobilized conditions, the actual immobilized HRP was 1.16 mg/g support, the activity of the immobilized HRP could reach as high as 1379.4 U/g support. Experiment results showed that the properties of storage stability, acid-base stability and the tolerance to the pH fluctuation of the immobilized HRP were better than those of the free HRP. The operation stability of the immobilized HRP was also good. The immobilized HRP is suitable for the oily wastewater treatment because of its reusability proved in this work 
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