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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la202703j
|2 doi
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|a pubmed24n0702.xml
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|a (NLM)21812484
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|a DE-627
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|e rakwb
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|a eng
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|a Hirtz, Michael
|e verfasserin
|4 aut
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|a Comparative height measurements of dip-pen nanolithography-produced lipid membrane stacks with atomic force, fluorescence, and surface-enhanced ellipsometric contrast microscopy
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|c 2011
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|a Text
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 13.01.2012
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|a Date Revised 13.09.2011
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2011 American Chemical Society
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|a Dip-pen nanolithography (DPN) with phospholipids has been shown to be a powerful tool for the generation of biologically active surface patterns, but screening of the obtained lithographic structures is still a bottleneck in the quality control of the prepared samples. Here we performed a comparative study with atomic force microscopy (AFM), fluorescence microscopy (FM), and surface-enhanced ellipsometric contrast (SEEC) microscopy of phospholipid membrane stacks consisting of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) with high admixing of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[6-[(2,4-dinitrophenyl)amino]hexanoyl] (DNP Cap PE) produced by DPN. We present a structural model of membrane stacking based on the combined information gained from the three microscopic techniques. Domains of phase-separated DNP Cap PE can be detected at high DNP Cap PE admixing that are not present at medium or low admixings. While the optical methods allow for a high-throughput screening of lithographic structures (compared to AFM), it was found that, when relying on FM alone, artifacts due to phase-separation phenomena can be introduced in the case of thin membrane stacks
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|a Comparative Study
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Phospholipids
|2 NLM
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|a Corso, Rémi
|e verfasserin
|4 aut
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|a Sekula-Neuner, Sylwia
|e verfasserin
|4 aut
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|a Fuchs, Harald
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 18 vom: 20. Sept., Seite 11605-8
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:27
|g year:2011
|g number:18
|g day:20
|g month:09
|g pages:11605-8
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|u http://dx.doi.org/10.1021/la202703j
|3 Volltext
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