Nonradioactive method to detect native single-stranded G-tails on yeast telomeres using a modified Southern blot protocol
Because of their low abundance and short length, telomeric single-stranded extensions have not traditionally been assessed by Southern blot analysis. Instead, most methods have relied on hybridizing radioactively labeled oligonucleotide probes to electrophoresed DNA within agarose gels. Here we desc...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 50(2011), 6 vom: 25. Juni, Seite 407-10
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| 1. Verfasser: |
Ortega, Lina M
(VerfasserIn) |
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| Weitere Verfasser: |
Hengartner, Christoph J,
Vega, Leticia R |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2011
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
DNA, Fungal
DNA, Single-Stranded
Oligonucleotide Probes
Digoxigenin
NQ1SX9LNAU |
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