Thickness profiling of formaldehyde-fixed cells by transmission-through-dye microscopy
Conventional light microscopy techniques are poorly suited for imaging the vertical cell dimension. This can be accomplished using transmission-through-dye (TTD) imaging, in which cell thickness is directly converted into image intensity in the presence of extracellular dye with strong absorption. W...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 50(2011), 6 vom: 25. Juni, Seite 389-96
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| 1. Verfasser: |
Pelts, Mariana
(VerfasserIn) |
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| Weitere Verfasser: |
Pandya, Sahil M,
Oh, Christine J,
Model, Michael A |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2011
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, N.I.H., Extramural
Fixatives
Fluoresceins
Fluorescent Dyes
Polymers
Formaldehyde
1HG84L3525
paraform
Y19UC83H8E |
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