Thickness profiling of formaldehyde-fixed cells by transmission-through-dye microscopy

Conventional light microscopy techniques are poorly suited for imaging the vertical cell dimension. This can be accomplished using transmission-through-dye (TTD) imaging, in which cell thickness is directly converted into image intensity in the presence of extracellular dye with strong absorption. W...

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Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 50(2011), 6 vom: 25. Juni, Seite 389-96
Auteur principal: Pelts, Mariana (Auteur)
Autres auteurs: Pandya, Sahil M, Oh, Christine J, Model, Michael A
Format: Article en ligne
Langue:English
Publié: 2011
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, N.I.H., Extramural Fixatives Fluoresceins Fluorescent Dyes Polymers Formaldehyde 1HG84L3525 paraform Y19UC83H8E