Thickness profiling of formaldehyde-fixed cells by transmission-through-dye microscopy
Conventional light microscopy techniques are poorly suited for imaging the vertical cell dimension. This can be accomplished using transmission-through-dye (TTD) imaging, in which cell thickness is directly converted into image intensity in the presence of extracellular dye with strong absorption. W...
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Détails bibliographiques
| Publié dans: | BioTechniques. - 1991. - 50(2011), 6 vom: 25. Juni, Seite 389-96
|
| Auteur principal: |
Pelts, Mariana
(Auteur) |
| Autres auteurs: |
Pandya, Sahil M,
Oh, Christine J,
Model, Michael A |
| Format: | Article en ligne
|
| Langue: | English |
| Publié: |
2011
|
| Accès à la collection: | BioTechniques
|
| Sujets: | Journal Article
Research Support, N.I.H., Extramural
Fixatives
Fluoresceins
Fluorescent Dyes
Polymers
Formaldehyde
1HG84L3525
paraform
Y19UC83H8E |