Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis

Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis

Copyright © 2011 Elsevier GmbH. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Journal of plant physiology. - 1979. - 168(2011), 17 vom: 15. Nov., Seite 2048-55
1. Verfasser: Okuma, Eiji (VerfasserIn)
Weitere Verfasser: Jahan, Md Sarwar, Munemasa, Shintaro, Hossain, Mohammad Anowar, Muroyama, Daichi, Islam, Mohammad Mahbub, Ogawa, Ken'ichi, Watanabe-Sugimoto, Megumi, Nakamura, Yoshimasa, Shimoishi, Yasuaki, Mori, Izumi C, Murata, Yoshiyuki
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Journal of plant physiology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Dinitrochlorobenzene Reactive Oxygen Species Abscisic Acid 72S9A8J5GW S-ethyl glutathione 7W95D60F4J Glutamate-Cysteine Ligase EC 6.3.2.2 mehr... Glutathione GAN16C9B8O Calcium SY7Q814VUP
Beschreibung
Zusammenfassung:Copyright © 2011 Elsevier GmbH. All rights reserved.
We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca(2+)-permeable channel currents by ABA or oscillation of the cytosolic free Ca(2+) concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca(2+) oscillation in ABA signal pathway of Arabidopsis guard cells
Beschreibung:Date Completed 03.03.2014
Date Revised 09.12.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1618-1328
DOI:10.1016/j.jplph.2011.06.002