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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2011.05.016
|2 doi
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|a pubmed24n0700.xml
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|a (DE-627)NLM209916524
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|a (NLM)21752490
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Borek, Sławomir
|e verfasserin
|4 aut
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|a Sucrose controls storage lipid breakdown on gene expression level in germinating yellow lupine (Lupinus luteus L.) seeds
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 03.05.2013
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2011 Elsevier GmbH. All rights reserved.
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|a This study revealed that cytosolic aconitase (ACO, EC 4.2.1.3) and isocitrate lyase (ICL, EC 4.1.3.1, marker of the glyoxylate cycle) are active in germinating protein seeds of yellow lupine. The glyoxylate cycle seems to function not only in the storage tissues of food-storage organs, but also in embryonic tissue of growing embryo axes. Sucrose (60mM) added to the medium of in vitro culture of embryo axes and cotyledons decreased activity of lipase (LIP, EC 3.1.1.3) and activity of glutamate dehydrogenase (NADH-GDH, EC 1.4.1.2). The opposite effect was caused by sucrose on activity of cytosolic ACO, ICL as well as NADP(+)-dependent (EC 1.1.1.42) and NAD(+)-dependent (EC 1.1.1.41) isocitrate dehydrogenase (NADP-IDH and NAD-IDH, respectively); activity of these enzymes was clearly stimulated by sucrose. Changes in the activity of LIP, ACO, NADP-IDH, and NAD-IDH caused by sucrose were based on modifications in gene expression because corresponding changes in the enzyme activities and in the mRNA levels were observed. The significance of cytosolic ACO and NADP-IDH in carbon flow from storage lipid to amino acids, as well as the peculiar features of storage lipid breakdown during germination of lupine seeds are discussed
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Amino Acids
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|a RNA, Messenger
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|a RNA, Plant
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|a Sucrose
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|a 57-50-1
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|a Carbon
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|a 7440-44-0
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|a Glutamate Dehydrogenase
|2 NLM
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|a EC 1.4.1.2
|2 NLM
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|a Lipase
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|a EC 3.1.1.3
|2 NLM
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|a Isocitrate Lyase
|2 NLM
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|a EC 4.1.3.1
|2 NLM
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|a Aconitate Hydratase
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|a EC 4.2.1.3
|2 NLM
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|a Nuc, Katarzyna
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 168(2011), 15 vom: 15. Okt., Seite 1795-803
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:168
|g year:2011
|g number:15
|g day:15
|g month:10
|g pages:1795-803
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|u http://dx.doi.org/10.1016/j.jplph.2011.05.016
|3 Volltext
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