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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la201898v
|2 doi
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|a eng
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| 100 |
1 |
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|a Bharti, Bhuvnesh
|e verfasserin
|4 aut
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| 245 |
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|a Aggregation of silica nanoparticles directed by adsorption of lysozyme
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 09.01.2012
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|a Date Revised 09.08.2011
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The interaction of the globular protein lysozyme with silica nanoparticles of diameter 20 nm was studied in a pH range between the isoelectric points (IEPs) of silica and the protein (pH 3-11). The adsorption affinity and capacity of lysozyme on the silica particles is increasing progressively with pH, and the adsorbed protein induces bridging aggregation of the silica particles. Structural properties of the aggregates were studied as a function of pH at a fixed protein-to-silica concentration ratio which corresponds to a surface concentration of protein well below a complete monolayer in the complete-binding regime at pH > 6. Sedimentation studies indicate the presence of compact aggregates at pH 4-6 and a loose flocculated network at pH 7-9, followed by a sharp decrease of aggregate size near the IEP of lysozyme. The structure of the bridged silica aggregates was studied by cryo-transmission electron microscopy (cryo-TEM) and small-angle X-ray scattering. The structure factor S(q) derived from the scattering profiles displays characteristic features of particles interacting by a short-range attractive potential and can be represented by the square-well Percus-Yevick potential model, with a potential depth not exceeding 3k(B)T
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Silicon Dioxide
|2 NLM
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|a 7631-86-9
|2 NLM
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|a Muramidase
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|a EC 3.2.1.17
|2 NLM
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| 700 |
1 |
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|a Meissner, Jens
|e verfasserin
|4 aut
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|a Findenegg, Gerhard H
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 16 vom: 16. Aug., Seite 9823-33
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|x 1520-5827
|7 nnns
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|g volume:27
|g year:2011
|g number:16
|g day:16
|g month:08
|g pages:9823-33
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