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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/err200
|2 doi
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|a pubmed24n1222.xml
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|a (NLM)21705387
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|a DE-627
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|e rakwb
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|a eng
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|a Lerich, Alexander
|e verfasserin
|4 aut
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|a Is the 6 kDa tobacco etch viral protein a bona fide ERES marker?
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 13.02.2012
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|a Date Revised 13.12.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The claim that the 6 kDa viral protein (VP) of Tobacco Etch Virus is a marker for ER exit sites (ERES) has been investigated. When transiently expressed as a CFP tagged fusion construct in tobacco mesophyll protoplasts, this integral membrane protein co-localizes with both the COPII coat protein YFP-SEC24 and the Golgi marker Man1-RFP. However, when over-expressed the VP locates to larger spherical structures which co-localize with neither ER nor Golgi markers. Nevertheless, deletion of the COPII interactive N-terminal D(X)E motif causes it to be broadly distributed throughout the ER, supporting the notion that this protein could be an ERES marker. Curiously, whereas brefeldin A (BFA) caused a typical Golgi-stack response (redistribution into the ER) of the VP in leaf epidermal cells, in protoplasts it resulted in the formation of structures identical to those formed by over-expression. However, anomalous results were obtained with protoplasts: when co-expressed with the non-cycling cis-Golgi marker Man1-RFP, a BFA-induced redistribution of the VP-CFP signal into the ER was observed, but, in the presence of the cycling Golgi marker ERD2-YFP, this did not occur. High resolution images of side-on views of Golgi stacks in epidermal cells showed that the 6 kDa VP-CFP signal overlapped considerably more with YFP-SEC24 than with Man1-RFP, indicating that the VP is proportionately more associated with ERES. However, based on a consideration of the structure of its cytoplasmic tail, the scenario that the VP collects at ERES and is transported to the cis-Golgi before being recycled back to the ER, is supported
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Biomarkers
|2 NLM
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| 650 |
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|a Viral Proteins
|2 NLM
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| 700 |
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|a Langhans, Markus
|e verfasserin
|4 aut
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|a Sturm, Silke
|e verfasserin
|4 aut
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| 700 |
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|a Robinson, David G
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 62(2011), 14 vom: 01. Okt., Seite 5013-23
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:62
|g year:2011
|g number:14
|g day:01
|g month:10
|g pages:5013-23
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|u http://dx.doi.org/10.1093/jxb/err200
|3 Volltext
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