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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2011.03780.x
|2 doi
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|a (NLM)21668885
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|a DE-627
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|e rakwb
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|a eng
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|a Tavares, Bárbara
|e verfasserin
|4 aut
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|a Calcium-regulated anion channels in the plasma membrane of Lilium longiflorum pollen protoplasts
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|c 2011
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 30.12.2011
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|a Date Revised 23.04.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.
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|a • Currents through anion channels in the plasma membrane of Lilium longiflorum pollen grain protoplasts were studied under conditions of symmetrical anionic concentrations by means of patch-clamp whole-cell configuration. • With Cl(-) -based intra- and extracellular solutions, three outward-rectifying anion conductances, I(Cl1) , I(Cl2) and I(Cl3) , were identified. These three activities were discriminated by differential rundown behaviour and sensitivity to 5-nitro-2-(phenylpropylamino)-benzoate (NPPB), which could not be attributed to one or more channel types. All shared strong outward rectification, activated instantaneously and displayed a slow time-dependent activation for positive potentials. All showed modulation by intracellular calcium ([Ca(2+) ](in) ), increasing intensity from 6.04 nM up to 0.5 mM (I(Cl1) ), or reaching a maximum value with 8.50 μM (I(Cl2) and I(Cl3) ). • After rundown, the anionic currents measured using NO(3) (-) -based solutions were indistinguishable, indicating that the permeabilities of the channels for Cl(-) and NO(3) (-) are similar. Additionally, unitary anionic currents were measured from outside-out excised patches, confirming the presence of individual anionic channels. • This study shows for the first time the presence of a large anionic conductance across the membrane of pollen protoplasts, resulting from the presence of Ca(2+) -regulated channels. A similar conductance was also found in germinated pollen. We hypothesize that these putative channels may be responsible for the large anionic fluxes previously detected by means of self-referencing vibrating probes
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Anions
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|a Chlorides
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|a Ion Channels
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|a Nitrates
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|a Nitrobenzoates
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|a Water
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|a 059QF0KO0R
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|a 5-nitro-2-(3-phenylpropylamino)benzoic acid
|2 NLM
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|a 3A35O9G3YZ
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|a Calcium
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|a SY7Q814VUP
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|a Dias, Pedro Nuno
|e verfasserin
|4 aut
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|a Domingos, Patrícia
|e verfasserin
|4 aut
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|a Moura, Teresa Fonseca
|e verfasserin
|4 aut
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|a Feijó, José Alberto
|e verfasserin
|4 aut
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|a Bicho, Ana
|e verfasserin
|4 aut
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|i Enthalten in
|t The New phytologist
|d 1979
|g 192(2011), 1 vom: 01. Okt., Seite 45-60
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
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|g volume:192
|g year:2011
|g number:1
|g day:01
|g month:10
|g pages:45-60
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|u http://dx.doi.org/10.1111/j.1469-8137.2011.03780.x
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