Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase α-subunit (ASA1) transcript and protein abundances an auxin-related mechanism for enhanced growth in GS transgenics?

Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase α-subunit (ASA1) transcript and protein abundances : an auxin-related mechanism for enhanced growth in GS transgenics?

© 2011 The Author(s).

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 62(2011), 13 vom: 19. Aug., Seite 4423-31
1. Verfasser: Man, Huimin (VerfasserIn)
Weitere Verfasser: Pollmann, Stephan, Weiler, Elmar W, Kirby, Edward G
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Indoleacetic Acids Protein Subunits RNA, Messenger Glutamine 0RH81L854J Anthranilate Synthase EC 4.1.3.27 Glutamate-Ammonia Ligase mehr... EC 6.3.1.2 Chorismic Acid GI1BLY82Y1
Beschreibung
Zusammenfassung:© 2011 The Author(s).
The initial reaction in the pathway leading to the production of indole-3-acetic acid (IAA) in plants is the reaction between chorismate and glutamine to produce anthranilate, catalysed by the enzyme anthranilate synthase (ASA; EC 4.1.3.27). Compared with non-transgenic controls, leaves of transgenic poplar with ectopic expression of the pine cytosolic glutamine synthetase (GS1a; EC 6.3.1.2) produced significantly greater glutamine and significantly enhanced ASA α-subunit (ASA1) transcript and protein (approximately 130% and 120% higher than in the untransformed controls, respectively). Similarly, tobacco leaves fed with 30 mM glutamine and 2 mM chorismate showed enhanced ASA1 transcript and protein (175% and 90% higher than controls, respectively). Furthermore, free IAA was significantly elevated both in leaves of GS1a transgenic poplar and in tobacco leaves fed with 30 mM glutamine and 2 mM chorismate. These results indicated that enhanced cellular glutamine may account for the enhanced growth in GS transgenic poplars through the regulation of auxin biosynthesis
Beschreibung:Date Completed 09.01.2012
Date Revised 20.03.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/err026