Nanogyroids incorporating multivalent lipids enhanced membrane charge density and pore forming ability for gene silencing

Nanogyroids incorporating multivalent lipids : enhanced membrane charge density and pore forming ability for gene silencing

© 2011 American Chemical Society

Bibliographische Detailangaben
Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 27(2011), 12 vom: 21. Juni, Seite 7691-7
1. Verfasser: Leal, Cecília (VerfasserIn)
Weitere Verfasser: Ewert, Kai K, Shirazi, Rahau S, Bouxsein, Nathan F, Safinya, Cyrus R
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Lipids RNA, Small Interfering
Beschreibung
Zusammenfassung:© 2011 American Chemical Society
The self-assembly of a custom-synthesized pentavalent cationic lipid (MVL5) and glycerol monooleate (GMO) with small interfering RNA (siRNA) results in the formation of a double-gyroid bicontinuous inverted cubic phase with colocalized lipid/siRNA domains as shown by synchrotron X-ray scattering and fluorescence microscopy. The high charge density (due to MVL5) and positive Gaussian modulus of the GMO-containing membranes confer optimal electrostatic and elastic properties for endosomal escape, enabling efficient siRNA delivery and effective, specific gene silencing
Beschreibung:Date Completed 28.09.2011
Date Revised 20.10.2021
published: Print-Electronic
Citation Status MEDLINE
ISSN:1520-5827
DOI:10.1021/la200679x