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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la200908h
|2 doi
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|a pubmed24n0695.xml
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|a (NLM)21598954
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Lehnert, Michael
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Adsorption and conformation behavior of biotinylated fibronectin on streptavidin-modified TiO(X) surfaces studied by SPR and AFM
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.09.2011
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2011 American Chemical Society
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|a It is well-known that protein-modified implant surfaces such as TiO(2) show a higher bioconductivity. Fibronectin is a glycoprotein from the extracellular matrix (ECM) with a major role in cell adhesion. It can be applied on titanium oxide surfaces to accelerate implant integration. Not only the surface concentration but also the presentation of the protein plays an important role for the cellular response. We were able to show that TiO(X) surfaces modified with biotinylated fibronectin adsorbed on a streptavidin-silane self-assembly multilayer system are more effective regarding osteoblast adhesion than surfaces modified with nonspecifically bound fibronectin. The adsorption and conformation behavior of biotinylated and nonbiotinylated (native) fibronectin was studied by surface plasmon resonance (SPR) spectroscopy and atomic force microscopy (AFM). Imaging of the protein modification revealed that fibronectin adopts different conformations on nonmodified compared to streptavidin-modified TiO(X) surfaces. This conformational change of biotinylated fibronectin on the streptavidin monolayer delivers a fibronectin structure similar to the conformation inside the ECM and therefore explains the higher cell affinity for these surfaces
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Fibronectins
|2 NLM
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|a titanium dioxide
|2 NLM
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|a 15FIX9V2JP
|2 NLM
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|a Biotin
|2 NLM
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|a 6SO6U10H04
|2 NLM
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|a Streptavidin
|2 NLM
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|a 9013-20-1
|2 NLM
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|a Titanium
|2 NLM
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|a D1JT611TNE
|2 NLM
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| 700 |
1 |
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|a Gorbahn, Miriam
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rosin, Christopher
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Klein, Marcus
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Köper, Ingo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Al-Nawas, Bilal
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Knoll, Wolfgang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Veith, Michael
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 12 vom: 21. Juni, Seite 7743-51
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:27
|g year:2011
|g number:12
|g day:21
|g month:06
|g pages:7743-51
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|u http://dx.doi.org/10.1021/la200908h
|3 Volltext
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