Leaf and root pectin methylesterase activity and 13C/12C stable isotopic ratio measurements of methanol emissions give insight into methanol production in Lycopersicon esculentum

© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 191(2011), 4 vom: 15. Sept., Seite 1031-1040
1. Verfasser: Oikawa, Patricia Yoshino (VerfasserIn)
Weitere Verfasser: Giebel, Brian M, da Silveira Lobo O'Reilly Sternberg, Leonel, Li, Lei, Timko, Michael P, Swart, Peter K, Riemer, Daniel D, Mak, John E, Lerdau, Manuel T
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Carbon Isotopes Carbon 7440-44-0 Pectins 89NA02M4RX Carboxylic Ester Hydrolases EC 3.1.1.- pectinesterase mehr... EC 3.1.1.11 Methanol Y4S76JWI15
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100 1 |a Oikawa, Patricia Yoshino  |e verfasserin  |4 aut 
245 1 0 |a Leaf and root pectin methylesterase activity and 13C/12C stable isotopic ratio measurements of methanol emissions give insight into methanol production in Lycopersicon esculentum 
264 1 |c 2011 
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500 |a Date Revised 07.12.2022 
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520 |a © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust. 
520 |a Plant production of methanol (MeOH) is a poorly understood aspect of metabolism, and understanding MeOH production in plants is crucial for modeling MeOH emissions. Here, we have examined the source of MeOH emissions from mature and immature leaves and whether pectin methylesterase (PME) activity is a good predictor of MeOH emission. We also investigated the significance of below-ground MeOH production for mature leaf emissions. We present measurements of MeOH emission, PME activity, and MeOH concentration in mature and immature tissues of tomato (Lycopersicon esculentum). We also present stable carbon isotopic signatures of MeOH emission and the pectin methoxyl pool. Our results suggest that below-ground MeOH production was not the dominant contributor to daytime MeOH emissions from mature and immature leaves. Stable carbon isotopic signatures of mature and immature leaf MeOH were similar, suggesting that they were derived from the same pathway. Foliar PME activity was related to MeOH flux, but unexplained variance suggested PME activity could not predict emissions. The data show that MeOH production and emission are complex and cannot be predicted using PME activity alone. We hypothesize that substrate limitation of MeOH synthesis and MeOH catabolism may be important regulators of MeOH emission 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Carbon Isotopes  |2 NLM 
650 7 |a Carbon  |2 NLM 
650 7 |a 7440-44-0  |2 NLM 
650 7 |a Pectins  |2 NLM 
650 7 |a 89NA02M4RX  |2 NLM 
650 7 |a Carboxylic Ester Hydrolases  |2 NLM 
650 7 |a EC 3.1.1.-  |2 NLM 
650 7 |a pectinesterase  |2 NLM 
650 7 |a EC 3.1.1.11  |2 NLM 
650 7 |a Methanol  |2 NLM 
650 7 |a Y4S76JWI15  |2 NLM 
700 1 |a Giebel, Brian M  |e verfasserin  |4 aut 
700 1 |a da Silveira Lobo O'Reilly Sternberg, Leonel  |e verfasserin  |4 aut 
700 1 |a Li, Lei  |e verfasserin  |4 aut 
700 1 |a Timko, Michael P  |e verfasserin  |4 aut 
700 1 |a Swart, Peter K  |e verfasserin  |4 aut 
700 1 |a Riemer, Daniel D  |e verfasserin  |4 aut 
700 1 |a Mak, John E  |e verfasserin  |4 aut 
700 1 |a Lerdau, Manuel T  |e verfasserin  |4 aut 
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773 1 8 |g volume:191  |g year:2011  |g number:4  |g day:15  |g month:09  |g pages:1031-1040 
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