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231224s2011 xx |||||o 00| ||eng c |
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|a 10.1021/la2015034
|2 doi
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|a pubmed24n0695.xml
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|a eng
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1 |
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|a Thilakarathne, Vindya
|e verfasserin
|4 aut
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|a Protein polymer conjugates
|b improving the stability of hemoglobin with poly(acrylic acid)
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|c 2011
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 28.09.2011
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|a Date Revised 25.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2011 American Chemical Society
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|a The synthesis, characterization, and evaluation of a novel polymer-protein conjugate are reported here. The covalent conjugation of high-molecular weight poly(acrylic acid) (PAA) to the lysine amino groups of met-hemoglobin (Hb) resulted in the covalent conjugation of Hb to PAA (Hb-PAA conjugate), as confirmed by dialysis and electrophoresis studies. The retention of native-like structure of Hb in Hb-PAA was established from Soret absorption, circular dichroism studies, and the redox activity of the iron center in Hb-PAA. The peroxidase-like activities of the Hb-PAA conjugate further confirmed the retention of Hb structure and biological activity. Thermal denaturation of the conjugate was investigated by differential scanning calorimetry and steam sterilization studies. The Hb-PAA conjugate indicated an improved denaturation temperature (T(d)) when compared to that of the unmodified Hb. One astonishing observation was that polymer conjugation significantly enhanced the Hb-PAA storage stability at room temperature. After 120 h of storage at room temperature in phosphate-buffered saline (PBS) at pH 7.4, for example, Hb-PAA retained 90% of its initial activity and unmodified Hb retained <60% of its original activity under identical conditions of buffer, pH, and temperature. Our conjugate demonstrates the key role of polymers in enhancing Hb stability via a very simple, efficient, general route. Water-swollen, lightly cross-linked, stable Hb-polymer nanogels of 100-200 nm were produced quickly and economically by this approach for a wide variety of applications
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Acrylic Resins
|2 NLM
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|a Hemoglobins
|2 NLM
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|a carbopol 940
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|a Briand, Victoria A
|e verfasserin
|4 aut
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|a Zhou, Yuxiang
|e verfasserin
|4 aut
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| 700 |
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|a Kasi, Rajeswari M
|e verfasserin
|4 aut
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| 700 |
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|a Kumar, Challa V
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 27(2011), 12 vom: 21. Juni, Seite 7663-71
|w (DE-627)NLM098181009
|x 1520-5827
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|g volume:27
|g year:2011
|g number:12
|g day:21
|g month:06
|g pages:7663-71
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|u http://dx.doi.org/10.1021/la2015034
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