Measuring adrenal autoantibody response interlaboratory concordance in the first international serum exchange for the determination of 21-hydroxylase autoantibodies

Measuring adrenal autoantibody response : interlaboratory concordance in the first international serum exchange for the determination of 21-hydroxylase autoantibodies

Copyright © 2011 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 140(2011), 3 vom: 15. Sept., Seite 291-9
1. Verfasser: Falorni, Alberto (VerfasserIn)
Weitere Verfasser: Chen, Shu, Zanchetta, Renato, Yu, Liping, Tiberti, Claudio, Bacosi, Maria Luisa, Furmaniak, Jadwiga, Bini, Vittorio, Dotta, Francesco, Eisenbarth, George S, Smith, Bernard Rees, Betterle, Corrado
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't Autoantibodies Biomarkers Steroid 21-Hydroxylase EC 1.14.14.16
Beschreibung
Zusammenfassung:Copyright © 2011 Elsevier Inc. All rights reserved.
21-hydroxylase autoantibodies (21OHAb) are the gold standard immune marker to identify patients with clinical or subclinical autoimmune Addison's disease (AAD). No assessment of interlaboratory concordance has been made for 21OHAb measurement. Serum samples from 267 patients with primary adrenal insufficiency and from 83 healthy control subjects were distributed to four independent laboratories that determined presence and titer of 21OHAb, by using radiobinding assays with either in vitro translated 35S-radiolabelled or 125I-radiolabelled autoantigen. Cohen's κ of inter-rater agreement ranged from 0.857 to 0.983, showing a very good concordance of the positive/negative score among the four laboratories. Passing-Bablok regression showed a good agreement of 21OHAb titers arranged by ranks, but important discrepancies emerged at the Bland-Altman plot, as the repeatability coefficient was much higher than the laboratory cut-offs, which indicates that results from different laboratories cannot be used interchangeably. A standardization international program for 21OHAb measurement is strongly needed
Beschreibung:Date Completed 19.10.2011
Date Revised 25.11.2016
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2011.04.012