Presence/absence, differential expression and sequence polymorphisms between PiAVR2 and PiAVR2-like in Phytophthora infestans determine virulence on R2 plants

Presence/absence, differential expression and sequence polymorphisms between PiAVR2 and PiAVR2-like in Phytophthora infestans determine virulence on R2 plants

© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 191(2011), 3 vom: 15. Aug., Seite 763-776
1. Verfasser: Gilroy, Eleanor M (VerfasserIn)
Weitere Verfasser: Breen, Susan, Whisson, Stephen C, Squires, Julie, Hein, Ingo, Kaczmarek, Maciej, Turnbull, Dionne, Boevink, Petra C, Lokossou, Anoma, Cano, Liliana M, Morales, Juan, Avrova, Anna O, Pritchard, Leighton, Randall, Eva, Lees, Alison, Govers, Francine, van West, Pieter, Kamoun, Sophien, Vleeshouwers, Vivianne G A A, Cooke, David E L, Birch, Paul R J
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2011
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Proteins Virulence Factors
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245 1 0 |a Presence/absence, differential expression and sequence polymorphisms between PiAVR2 and PiAVR2-like in Phytophthora infestans determine virulence on R2 plants 
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500 |a Date Revised 08.04.2022 
500 |a published: Print-Electronic 
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520 |a © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust. 
520 |a • A detailed molecular understanding of how oomycete plant pathogens evade disease resistance is essential to inform the deployment of durable resistance (R) genes. • Map-based cloning, transient expression in planta, pathogen transformation and DNA sequence variation across diverse isolates were used to identify and characterize PiAVR2 from potato late blight pathogen Phytophthora infestans. • PiAVR2 is an RXLR-EER effector that is up-regulated during infection, accumulates at the site of haustoria formation, and is recognized inside host cells by potato protein R2. Expression of PiAVR2 in a virulent P. infestans isolate conveys a gain-of-avirulence phenotype, indicating that this is a dominant gene triggering R2-dependent disease resistance. PiAVR2 presence/absence polymorphisms and differential transcription explain virulence on R2 plants. Isolates infecting R2 plants express PiAVR2-like, which evades recognition by R2. PiAVR2 and PiAVR2-like differ in 13 amino acids, eight of which are in the C-terminal effector domain; one or more of these determines recognition by R2. Nevertheless, few polymorphisms were observed within each gene in pathogen isolates, suggesting limited selection pressure for change within PiAVR2 and PiAVR2-like. • Our results direct a search for R genes recognizing PiAVR2-like, which, deployed with R2, may exert strong selection pressure against the P. infestans population 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Proteins  |2 NLM 
650 7 |a Virulence Factors  |2 NLM 
700 1 |a Breen, Susan  |e verfasserin  |4 aut 
700 1 |a Whisson, Stephen C  |e verfasserin  |4 aut 
700 1 |a Squires, Julie  |e verfasserin  |4 aut 
700 1 |a Hein, Ingo  |e verfasserin  |4 aut 
700 1 |a Kaczmarek, Maciej  |e verfasserin  |4 aut 
700 1 |a Turnbull, Dionne  |e verfasserin  |4 aut 
700 1 |a Boevink, Petra C  |e verfasserin  |4 aut 
700 1 |a Lokossou, Anoma  |e verfasserin  |4 aut 
700 1 |a Cano, Liliana M  |e verfasserin  |4 aut 
700 1 |a Morales, Juan  |e verfasserin  |4 aut 
700 1 |a Avrova, Anna O  |e verfasserin  |4 aut 
700 1 |a Pritchard, Leighton  |e verfasserin  |4 aut 
700 1 |a Randall, Eva  |e verfasserin  |4 aut 
700 1 |a Lees, Alison  |e verfasserin  |4 aut 
700 1 |a Govers, Francine  |e verfasserin  |4 aut 
700 1 |a van West, Pieter  |e verfasserin  |4 aut 
700 1 |a Kamoun, Sophien  |e verfasserin  |4 aut 
700 1 |a Vleeshouwers, Vivianne G A A  |e verfasserin  |4 aut 
700 1 |a Cooke, David E L  |e verfasserin  |4 aut 
700 1 |a Birch, Paul R J  |e verfasserin  |4 aut 
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