|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM207784914 |
| 003 |
DE-627 |
| 005 |
20231224002826.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2011 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1107/S0909049511007631
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0693.xml
|
| 035 |
|
|
|a (DE-627)NLM207784914
|
| 035 |
|
|
|a (NLM)21525640
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Juers, Douglas H
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Similarities and differences in radiation damage at 100 K versus 160 K in a crystal of thermolysin
|
| 264 |
|
1 |
|c 2011
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 16.08.2011
|
| 500 |
|
|
|a Date Revised 28.04.2011
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status PubMed-not-MEDLINE
|
| 520 |
|
|
|a The temperature-dependence of radiation damage in macromolecular X-ray crystallography is currently much debated. Most protein crystallographic studies are based on data collected at 100 K. Data collection at temperatures below 100 K has been proposed to reduce radiation damage and above 100 K to be useful for kinetic crystallography that is aimed at the generation and trapping of protein intermediate states. Here the global and specific synchrotron-radiation sensitivity of crystalline thermolysin at 100 and 160 K are compared. Both types of damage are higher at 160 K than at 100 K. At 160 K more residue types are affected (Lys, Asp, Gln, Pro, Thr, Met, Asn) than at 100 K (Met, Asp, Glu, Lys). The X-ray-induced relative atomic B-factor increase is shown to correlate with the proximity of the atom to the nearest solvent channel at 160 K. Two models may explain the observed correlation: either an increase in static disorder or an increased attack of hydroxyl radicals from the solvent area of the crystal
|
| 650 |
|
4 |
|a Journal Article
|
| 700 |
1 |
|
|a Weik, Martin
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 18(2011), Pt 3 vom: 18. Mai, Seite 329-37
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
|
| 773 |
1 |
8 |
|g volume:18
|g year:2011
|g number:Pt 3
|g day:18
|g month:05
|g pages:329-37
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1107/S0909049511007631
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_40
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_2005
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 18
|j 2011
|e Pt 3
|b 18
|c 05
|h 329-37
|